Background:The parasitic infection may be caused by the parasites Trichomonas tenax and Entamoeba gingivalis, which were included in this investigation. This studys goal is to estimation the incidence the parasites in patients' oral cavities with associated of some factors like residency, dental caries and diabetics in Erbil, Iraq.Materials and Methods:The current study comprised 354 patients in total. A questionnaire recorded residency (urban and Rural), dental caries and diabetics. Calculi samples were taken by use of a sterile curette and were placed in sterile collection vials, each containing 1 ml of sterile normal saline solution. Saliva samples of the patients were collected in sterile vials, then dilution was done by addition of 1 ml of sterile saline solution to each vial. Then each vial was labeled by patient’s number, name and date of collection. The purpose of collection the calculi and saliva to search about the parasites in the oral cavity. Results:The higher infection ratio of E. gingivalis in the rural regions in comparison with that of urban areas (91.7% and 89% respectively), and the results discovered that the higher positive rate of E. gingivalis (93.3%) was observed among those with dental caries and it was encountered in (86.8%)of subjects free from dental caries, regarding diabetes, diabetes and the presence of E. gingivaliswere shown to have a statistically significant connection (97.1%) and (35.7%) infection rates of E. gingivalis among diabetics and non-diabetic participitants respectively. While T. tenax was more common in individuals with non-diabetes (12.2%) compare to the diabetics (9.5%), but no relation was found.Conclusion:Infection with these parasites is relatively prevalent in people with dental caries, diabetics, and the persons' environment. It appears that continuation of advices are important in regulator of parasitic infection in Kurdistan Region of Iraq.
Pediculosis is an integumentary disease caused by the ecto-parasite Pediculus humanus capitis, which infests human hair. It is a common public health problem that is most prominent worldwide in elementary school children. The current study aimed to investigate the prevalence, risk factors, and genetic diversity of P. humanus capitis among primary school children in the Erbil province. For this purpose, this study was conducted from October 2019 to December 2019 among 1100 randomly selected elementary school children aged 6-12. Data collection was performed via a regular questionnaire and physical hair examination. For the genetic diversity part, after collecting one louse randomly from each individual, DNA was extracted. The mitochondrial Cox1 gene was then amplified by universal primer and PCR. Gene sequencing was performed by ABI (BioNEER, South Korea). Data analysis was done by Chi-Square and T-test using the SPSS ver. 23. The overall infestation rate was 21.27%, and the rate was significantly higher among females (34.93%) compared to males (7.91%). Some variables had found the prevalence rate to be strongly affected. This included age; the rate was not significant (26.87%) in the age group 8-9 years compared to other age groups. According to hair length, the rate was significantly increased (36.52%) among children with tall hair. In terms of hair type, the incidence of curly-haired children was significantly higher (31.54%); in terms of hair color, there were not significant differences among blonde children (25.90%) and others. According to the results of Cox1 gene sequencing, of 234 infested children to lice, 86 (36.75%) of them were exposed to clade A, 38 (16.24%) were exposed to clade B, clade C has not been seen among any children (0%), 105 students (44.87%) were exposed to clade D, and 5 of them exposed to clade E (2.14%). Eventually, a significantly higher incidence (33.78%) was reported in rural primary school children. The infection rate of human head lice in Erbil province is still high, which is one of the health problems of children in public schools.
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