No abstract

The present study has been carried out at the College of Veterinary Medicine ,AL-Qadisiya University to determine the effect of obesity on serum Estrogen concentration in mature female Wistar rats.Fifty immature female rats (14-20 days age,30-40g.) were housed at the animal house during the period extended from 25th March,2011 to 10th June,2011.Animals were randomly divided into two equal groups. Control fed on standard feed and treated fed on high fat feed ( 32.3%fat by weight and 4828 k cal/kg energy). Daily body weights were recorded during the experiment period extended to the 100th day of age .At 60 days of age ,eight of mature female Wistar rats from each group were anesthetized, dissected and blood samples were obtained from abdominal vein for assessment of Estrogen concentration in serum. Ovaries and uteri were also obtained, weighted and fixed in formalin 10% for histopathological study.Ovarian and uterine weights of treated group recorded significant decrement in comparison with that of control.Hormonal assay in sera showed significant decrement of estrogen in treated mature female rats in comparison with that of control female rats. Ovarian sections of treated female rats revealed lower level of proliferation in the follicular tissue and predominance of Graffian follicles compared with control. Similarly uterine sections of treated female rats showed filtration of adipose tissue with less proliferated changes and less uterine glands compared with that in control.

The current study was carried to investigate the effect of green tea extract in improvement of male rats reproductive efficiency after exposed to oxidative stress by streptozotocin.40 male rats at 60 days old with 135±11gm in weight were randomly divided into four equal groups, the first drenched with distilled water for 60 days as control group(C).the second (T1)was given aqueous extract of green tea (100mg/kg/bw) for 60 days, while the third group (T2 injected (i.p) single dose of streptozotocin (60mg/km/bw) for induction of oxidative stress, the forth group injected with single dose of streptozotocin (60mg/km/bw) and after 30 days drenched with green tea extract(100mg/km/bw) for 30 days.at day60 of experiment all animals were sacrificed , blood samples were collected from ventral vein and serum samples were isolated for measurement of male reproductive hormones (LH,FSH, and Testosterone) by ELISA test. the testes samples were taken for histological study and dimensions of seminiferous tubules, samples of epididymis for study of seminiferous tubules dimensions. The results of study was revealed significant increase (P≥0.05)in testosterone and FSH in (T1) group compared with other groups while there is non-significant changes in LH concentration compared with other groups.

This study was conducted to evaluate the effectiveness of the CoQ10 on the reproductive system of female rats and it's ability to reduce the harmful effect of lead acetate. To achieve this goal an experiment was done on 24 mature female rats which are randomly divided into 4 groups, group(1) treated with 1ml/kg corn oil and considered as a negative control group,  group(2) treated with CoQ10  at dose 200 mg/kg and considered as a positive control group,  group(3) treated with lead acetate at dose 8mg /kg ,and  group (4) treated with lead acetate for 30 days then after CoQ10  for another 30 days. Blood samples were taken from all animals after 60 days for biochemical analysis to estimate FSH, LH, estrogen and progesterone hormones .Histological examinations of an ovary and uterus were also  involved  in this study. Results showed a significant increase (p< 0.05) in serum FSH, estrogen and progesterone levels  in group(2) compared to other  groups  while LH level was  significantly increased (p< 0.05) compared to group (3). Group( 3) showed a significant decrease (p< 0.05) in serum FSH, LH level  in compared to group (1) and group( 4 ),while progesterone and estrogen levels showed no significant difference (p> 0.05) compared to group(1) . At the same time, there were no significant difference (p> 0.05) in serum LH, estrogen and progesterone levels  in group (4) compared to group(1), while there was a significant decrease (p< 0.05) in serum FSH level  in group(4) compared group(1). The results of ovary sections ,group( 2)  showed  normal structure and distribution pattern of various ovary components, while group (3)showed sever hemorrhage in the ovarian stroma and  suppression of ovarian follicles. Group(4) showed particular recovery in ovarian parenchyma. Uterus sections of group(2) showed normal epithelium which lining the uterus and there is destitution uterine glands. Group(3) showed epithelium degenerative, small and non developed uterine glands and hemorrhage in uterine tissue .Other wised uterus in group (4)showed normal columnar epithelium which lining the uterus, and proliferation of uterine glands.

This study was carried out to determine the changes in oxidant – antioxidant status and some biochemical parameters in pregnant Iraqi female camels in comparison with non pregnant one. To determine this objective (30) blood samples from pregnant female camels and (30) blood samples from non pregnant female camels was taken at last state of pregnancy (number of animals are 30 in each group) , blood samples were put in the test tubes contain anticoagulant (EDTA) , after centrifugation serum samples were taken for measurement of Malondialdehyde (MDA) , albumin , uric acid , total protein , alkaline phosphatase and urea concentrations.The results revealed significant increase (P < 0.05) in malon dialdehyde concentration in pregnant female camels in comparison with non pregnant. Also the results were showed insignificant decrease in albumin concentration and significant increase (P <0.05) in uric acid concentration in pregnant group which may be due to its antioxidant activity against lipid peroxidation during pregnancy. In related to the biochemical parameters the results were showed significant decrease (P <0.05) in the total protein of pregnant female camels which may be due to decrease in albumin concentration, while there is significant increase (P <0.05) in ALP.