The aim of this study was to extract peroxides enzyme from Peganum harmala seeds; peroxides was extracted by using different extraction buffer solutions, then it was purified by three steps of purification includes precipitation with ammonium sulfate in a saturation ratio of 70 %, ion exchange chromatography through DEAE-Cellulose, and gel filtration chromatography throughout Sephadex G-100, and determine the optimum condition for extraction. This was performed by controlling the type and concentration of buffer, pH of the buffer used, and the ratio of extraction. The Sodium acetate buffer with 0.2mM and pH 5.0 was found to be the best buffer for the extraction of peroxidase. By using the extraction ratio for a plant of 1:3 (W/V), the specific activity was 195 U/mg protein. These three purification steps raised the specific activity to 235U/mg protein in the precipitation step with purification fold 2.3 and enzyme recovery 69%; the specific activity was increased to 243U/mg protein in Ion exchange step with purification fold 2.4 and enzyme recovery 23%, also the specific activity doubled after gel filtration step to 447U/mg protein with purification fold 4.4 and enzyme recovery 15%.

The aim of the present study is to examine the effect of (Chloroquine CQ) on the oxidative stress under water stress effect Recently, this compound is used to treat COVID-19, Hydrogen peroxide H2O2 effect and Stimulate the enzymatic system through the exposure of the plant to water stress and interaction with Chloroquine, where the stress stimulate the production of these Oxidative Factors (Hydrogen Peroxide, Malondialdehyde(MDA), Protease increase the production of these oxidative factors increase activity through the effect of Chloroquine, This process was performed in the meristematic tissues of the cells of the permanent Mung bean (Vigna radiata) plants under experimental factors (water stress factor for 10 days with control period of 5 days) (Chloroquine factors and concentrations of 125 and 250 mg.L-1 with control treatment) were designed using a Factorial Randomized Block Design (R.B.C.D) with three replicates and 18 experimental units The experimental unit area was 2 m. The results have shown the following; The water stress period of 10 days led to the production of toxic hydrogen peroxide and increased concentration by stress. The effect of water stress was Stimulate the Production of hydrogen peroxide in Meristematic tissues. The effect of Chloroquine CQ with increased concentrations had a role in inhibiting the production of enzymatic antioxidants (Superoxide dismutase(SOD) and Catalase (CAT). The interaction between water stress and increased Chloroquine CQ concentrations has been instrumental in stimulating the production of oxidative Factors.

An plastic pots experiment was conducted, in (the Botanical Garden of Biology Department in the Faculty of Education Ibn Al-Haytham- University of Baghdad), for a growing season(2019-2020), in order to study the effect of phosphate rocks, the level of addition and non-addition, the last is the control treatment, and spraying with kinetin, In three concentrations(25, 50, 100) mg. L −1 as well as control treatment it’s do not spray, and the their interactions on the content of: nitrogen, phosphorus, potassium, chlorophyll and concentration: protein and carbohydrates in the shoot of basil. The results confirmed that the addition of phosphate rock and sprinkling of kinetin and its interference have a significant effect on the studied traits of basil. The treatment of adding the phosphorous rock and the concentration 100 mg. L −1 gave in the highest values for the above mentioned characteristics.