Many typical liquefaction remediation techniques are not appropriate for application under existing buildings and more novel techniques are required. This paper describes centrifuge tests investigating the performance of cementation as a liquefaction remediation method. Two soil profiles with the same superstructure were tested under earthquake shaking. The first profile consisted of a deep layer of loose, liquefiable sand. The second comprised a shallow layer of loose sand overlying dense sand. Centrifuge tests were carried out with a cemented zone underneath the structure, through the full depth of the liquefiable layers and also partial depth. The superstructure was modelled as a single-degree-offreedom system. It is found that a cemented zone through the full depth of a liquefiable layer results in considerable reduction of structural settlements. Increased magnitude and higher frequency accelerations are transmitted to the structure but, depending on the building characteristics, it is likely that improved overall seismic performance can be achieved. Improvements in structural settlements can also be obtained with partialdepth remediation, if the depth of the cemented zone is greater than the depth of liquefaction. This type of remediation seems to have little effect on the accelerations transmitted to the structure.

The ground is a natural grand system; it is composed of myriad constituents that aggregate to form several geologic and biogenic systems. These systems operate independently and interplay harmoniously via important networked structures over multiple spatial and temporal scales. This paper presents arguments and derivations couched by the authors, to first give a better understanding of these intertwined networked structures, and then to give an insight of why and how these can be imitated to develop a new generation of nature-symbiotic ground engineering techniques. The paper draws on numerous recent advances made by the authors, and others, in imitating forms (e.g. synthetic fibres that imitate plant roots), materials (e.g. living composite materials, or living soil that imitate fungi and microbes), generative processes (e.g. managed decomposition of construction rubble to mimic weathering of aragonites to calcites), and functions (e.g. recreating the self-healing, selfproducing, and self-forming capacity of natural systems). Advances are reported in three categories of Materials, Models, and Methods (3Ms). A novel value-based appraisal tool is also presented, providing a means to vet the effectiveness of 3Ms as standalone units or in combinations.

In this paper, we describe the first steps in the design of a synthetic biological system based on the use of genetically modified bacteria to detect elevated pressures in soils and respond by cementing soil particles. Such a system might, for example, enable a self- constructed foundation to form in response to load using engineered bacteria which could be seeded and grown in the soils. This process would reduce the need for large-scale excavations and may be the basis for a new generation of self-assembling and responsive bio-based materials. A prototype computational model is presented which integrates experimental data from a pressure sensitive gene within Escherichia coli bacteria with geotechnical models of soil loading and pore water pressure. The results from the integrated model are visualised by mapping expected gene expression values onto the soil volume. We also use our experimental data to design a two component system where one type of bacteria acts as a sensor and signals to another material synthesis bacteria. The simulation demonstrates the potential of computational models which integrate multiple scales from macro stresses in soils to the expression of individual genes to inform new types of design process. The work also illustrates the combination of in silico (silicon based computing) computation with in vivo (in the living) computation.

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Hydrostatic pressure is an important physical stimulus which can cause various responses in bacterial cells. The survival and cellular processes of Escherichia coli under hydrostatic pressures between 10 MPa and 110 MPa have been studied. However, understanding bacterial responses to moderately elevated pressure of up to 10 MPa is useful for a range of different applications including for example in smart and responsive materials. In this study, the genetic responses of E. coli K-12 MG1655 to 1 MPa pressure was examined using transcriptomic analysis by RNA-Seq. The results show that 101 genes were differentially expressed under 1 MPa pressure in E. coli cells, with 85 of them up-regulated. The analysis suggested that some genes were over expressed to adapt the increase of oxygen levels in our system, and several functional categories are involved including oxidative stress responses, Fe-S cluster assembly and iron acquisition. Two differentially expressed genes azuC and entC were further investigated using RT-qPCR, and GFP reported strains of those two genes were created, AG1319 (PazuC azuC-msfgfp) and AG1321 (PentC entC-msfgfp). A linear response of azuC expression was observed between 0 MPa to 1 MPa by monitoring the fluorescence signal of strain AG1319 (PazuC azuC-msfgfp). This study is the first report to demonstrate the genetic response of bacterial cells under 1 MPa hydrostatic pressure, and provides preliminary data for creating pressure sensing bacterial strains for a wide range of applications.