Helena Paula Viaro scite author profile

We designed a primer pair (BtubNomF/BtubNomR) specifically for amplifying Aspergillus nomius DNA. In vitro assays confirmed BtubNomF/BtubNomR specificity, corroborating its usefulness in detecting and identifying A. nomius. We then investigated the occurrence of A. nomius in floral visitors of Bertholletia excelsa trees by means of PCR, and A. nomius was detected in the following bees: Xylocopa frontalis, Bombus transversalis, Centris denudans, C. ferruginea, and Epicharis flava. The presence of A. nomius in bees visiting Brazil nuts opens up new avenues for obtaining novel insights into the process whereby Brazil nuts are contaminated by aflatoxin-producing fungi.

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Fungi in Grapes Cultivated in Brazil Used for Juice Production

Ferranti¹,

Fungaro²,

Taniwaki³

et al. 2014

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Identification of Aspergillus Nomius in Floral Visitors of Brazil Nuts

Massi¹,

Penha²,

Casimiro³

et al. 2014

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