Contamination of ration components and roughage fed to dairy cows and finishing bulls in two different agricultural ecosystems in the district of Uherské Hradi‰tû (localities Buchlovice and Stupava) was monitored. Whereas the site Buchlovice was characterised by intensive agricultural production, the site Stupava was located in the protective zone of the Koryãany water reservoir and ecological agriculture was practised in a part of this area. Materials used for analyses included irrigation water, grass growth, haylage, wheat, roughage, preserved feeds and trough samples of feed. Samples of alfalfa and clover were also collected as ration components and bioindicators of plant origin. From each sample kind and/or sampling site 15 samples were collected. In addition to feeds, bovine milk and beef liver and muscle (m. longissimus dorsi) samples were analysed (10 samples of animal material). Imissions were measured by the mobile laboratory of Regional Hygienic Services, Brno, allowing the determination of SO 2 , NO x , CO, O 3 , fly ash. The samples were analysed for polychlorinated biphenyls (PCB) by high resolution gas chromatography with electron capture detection. Also tested for PCB residues were milk and tissue samples collected from dairy cows and bulls. The concentrations of indicator PCB congeners did not exceed the safety limit in any of the sampling areas (mean concentrations of PCB in feed between 1-2 µg·kg -1 ; mean concentration of PCB and animal tissues between 2-9 µg·kg -1 ). Calculation of the transfer coefficient Q proved to be a suitable tool for the assessment of residue transfer (dairy cows 0.2-1.0; bulls 0.3-1.0). Feed was identified as an important contamination source for animal tissues and milk. Polychlorinated biphenyls, agricultural ecosystem, transfer coefficient Q, food chainsLike other ecosystems, the agricultural ecosystem is exposed to the action of contaminating xenobiotics. In accordance with the world-wide tendencies, criteria for the assessment of the burden by xenobiotics are currently tightened in the Czech Republic to prevent possible damage to human and animal health. Also significant are the effects of pollutants on ecological aspects of the agricultural landscape.In addition to some extrinsic factors of agricultural ecosystems, the transfer of chemical xenobiotics in food chains depends also on intrinsic factors of the agricultural production. The extrinsic factors include the pollution of air, dry and wet imissions, and surface water; the intrinsic factors are associated with biological, physical, chemical and biological characteristics of soil, with the technology of agricultural production, and with zoohygienic conditions. The intrinsic factors become more significant in areas with intensive agricultural production, in which ecological dysbalance of the agricultural ecosystem often develops. Its major cause are high concentration of farm animals, inadequate handling of wastes from animal production, and excessive use of fertilisers. Such activities result in a complex soil...

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There was performed test of ecotoxicity with using earthworm Eisenia Fetida as chief representative of terrestrial organisms in the study. Soil matrix LUFA 2.3 was selected as a model of natural soil environment. Tested FEAs were follows: Sthamex F-15, Moussol-APS F-15 and Finiflam F-15. For experimental purposes, laboratory installation for infiltration was designed and compiled. Infiltration of three FEAs solutions was done before the ecotoxicity testing. Contaminated soil after infiltration was tested for semi-chronic ecotoxicity and reproduction ecotoxicity then. Introductory results indicates differences between each FEA, caused by various composition. Sthamex F-15 was determined as the most ecotoxic agent at all. Whilst Moussol-APS F-15 was evaluated as the agent with least ecotoxicity effect.

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