Over 40% of the world's population is under danger from mosquito transmitted diseases, which are a growing global health challenge. Since 2000, malaria control has made significant progress, but of late it has slowed down. Aedes-borne arbovirus risk is also rising quickly due to the remarkable expansion of the dengue and chikungunya viruses, yellow fever outbreaks, and the Zika virus pandemic. To counteract this issues people used many vector control measures. Synthetic pesticides play a significant role in mosquito larval and adult control strategies, although their deployment is hampered by the development of resistance. As an alternative, various biological measures are being used including bacterial bio pesticides. They are extremely effective at controlling larvae due to their lack of resistance development and environmental friendliness. In this present review various biological control measures used for mosquito control are discussed.

Mosquito-borne diseases are illnesses caused by the transmission of viruses, parasites, and protozoa to humans by mosquito vectors. Mosquitoes spread various diseases, including, dengue fever, malaria, chikungunya, West Nile fever, Zika and yellow fever. Bacterial DNA and cellular proteins were shown to help produce AgNPs, with bacterial DNA acting as a stabilising agent and cellular proteins acting as both a reducing and stabilising agent. As a result, the bacterial lysate, which contains a lot of DNA and proteins, could help in the creation of AgNPs that are larvicidal against mosquito larvae. Therefore, this work was aimed to study the larvicidal effect of synthesized AgNPs by Bacillus cereus VCRC 641 cell lysate. The present study demonstrates the larvicidal efficacy of silver nanoparticles (AgNPs) produced by Bacillus cereus VCRC 641 against the diseases-causing mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi. Silver nano particles were synthesized using Bacillus cereus to enhance its mosquitocidal efficacy. The level of toxicity in the AgNPs synthesized B. cereus was increased 117 fold in comparison with the cell lysate.

Vector borne diseases are increasing nowadays and can spread to new locations easily. Diseases like zika virus fever, West Nile fever, malaria, yellow fever, Japanese encephalitis, dengue, etc. are examples of mosquito-borne diseases. These diseases are majorly caused by three species of mosquitoes Anopheles, Aedes, and Culex. Most mosquito vector control methods depend upon outdoor spraying, impregnated nets, or indoor residual spraying of chemical insecticides and it was observed that many mosquito species evolved resistance to major pesticide classes. The disadvantages of using chemical pesticides are the development of resistance in mosquitoes and also the harm it causes to other non-target organisms, in addition to the higher cost, more labor, and other drawbacks. Using DEET is also not as preferable as it can induce muscle twitching, seizures, slurred speech, nausea, and rashes. DEET will not give long-term protection against all malaria-causing mosquito species, such as Anopheles. Whereas biocontrol agents used for mosquito control show no environmental pollution or resistance. Bacillus thuringiensis israelensis and Bacillus sphaericus biopesticides tend to remain longer in the environment, particularly in dirty water, and hence may be a viable choice for long-term mosquito control. They have the least adverse effect on living things like humans, domestic animals, and wildlife.

Mosquitoes are most important insect vectors for human disease transmission. Dengue, yellow fever, chikungunya, and zika are all spread by Aedes mosquitoes in many parts of the world. The primary vector of human malaria transmission is Anopheles. Culex is responsible for the transmission of Japanese encephalitis, lymphatic filariasis and West Nile virus fever. Synthetic pesticides have been successfully utilized in mosquito-control programmes for several decades. However, there are a number of disadvantages to the chemical method. As a result, there has been a rise in concern in the usage of biological agents for mosquito control in recent years. Because of their highly effective mosquitocidal activity, Bacillus sphaericus and Bacillus thuringiensis israelensis have been utilized extensively. While insecticide-based interventions have successfully reduced mosquito populations for a number of years, the reliance on a few number of insecticides has now led to the evolution of resistance. So there is a need to search for novel mosquitocidal bacteria for control the mosquito species. Since soil is one of the world's most diversified environments in which bacteria and fungi are abundant, screening of soil samples for isolation of novel mosquitocidal bacteria is attempted.

It is a known fact that edible aquatic animals possess a rich source of minerals and metallic compounds. In order to analyze the composition of minerals and heavy metals (Zn, Cu, Fe, Mn, Cd, Pb, and Cr) present in these fauna, this study was conducted in two fishes mullet and cat fish (Mugil cephalus& Ictalurus punctatus), two crustaceans prawn and crab (Fenneropenaeus indicus& Portunus sanguinolentus) and mussel (Perna viridis) which were collected from Thengaithittu estuary region, Puducherry, India. The results showed that the Zinc concentration is maximum in prawn and minimum in catfish, Copper concentration is maximum in catfish and minimum in mullet fish, Iron concentration is higher in catfish and minimum in mussel, Manganese concentration is maximum in mussel and minimum in prawn, Cadmium concentration is higher in crab and minimum in mussel, lead content is maximum in mullet and minimum in crab and Chromium concentration is higher in mussel and minimum in mullet. Mineral analysis reveals that the prawn had the good source of sodium, potassium and calcium and least in mussel. In this context the heavy metals and minerals concentration in estuarine fish, prawn, crab and mussel perceived in the current study was within the tolerable limits. The results of the current study suggest that eating fish, prawns, crab, and mussels from the Thengaithittu estuary in Puducherry may not have any negative effects.