Abstract. Lestari DA, Azrianingsih R, Hendrian H. 2017. Taxonomical position of Annonaceae species from East Java, Indonesia: collections of Purwodadi Botanic Garden based on morphological character. Biodiversitas 18: 1067Biodiversitas 18: -1076. Morphological characters are one of the most consistent of taxonomical markers are used in Annonaceae today. One of the plants collections from Annonaceae species which came from East Java and conserved in Purwodadi Botanic Garden-Indonesian Institute of Sciences, Purwodadi, and East Java has some problems in morphological characters. It has some problems that causing the taxonomical position of these species are not clear, i.e. presence of unidentified plant until level species. The aim of this study is to classify and identify of species that have not been identified in order to taxonomical position be clearly. Material samples are used Annonaceae collection from East Java and Magnoliaceae as out-group. Samples of these plants were observed qualitatively and quantitatively through morphological characters were analyzed using cluster analysis and then synapomorphy, autapomorphy and apomorphy character analysis to identify and to determine the taxonomical position. The results showed that there are 4 tribes and two sub-families in the dendrogram. Tribe includes Miliuseae, Xylopiae, Annoneae and Uvariae, as well as sub-family of Malmeoideae and Annonoideae. Sub-family distinguished by inner petal and habit, sub-family of Malmeoideae has connate inner petals and trees habit, sub-family of Annonoideae has free inner petal and woody climber habit, some of the trees. Species are unidentified suspected as a different species based on the proximity of group formed. There are five plant species that have not been identified to the species level. These species can be recommended the taxonomical position and proposed of species name through dendrogram with the name of the specimen. Specimen 1 is predicted to have close relations with Annona muricata, specimen 2 is predicted to have close relations with Miliusa macropoda, specimen 3 is predicted to have close relations with Artabotrys uncinatus and identified as Uvaria micrantha, specimen 4 is predicted to have close relations with Fissistigma latifolium and specimen 5 is predicted to have close relations with Saccopetalum horsfieldii, because these species are in one group.
Starch is regarded as vital in the food industry, where granule size and shape determine its function and uses. One plant taxon that is widely known as a starch source is Dioscorea (Dioscoreaceae), whose starch and protein content make it as healthy food. As the initial step to identify which varieties possess potential as starch sources, we conducted the study on starch granule morphology of some local varieties of D. hispida and D. alata. The aim of this study was to determine the shape and size variation of the starch granule of each variety of D. hispida and D. alata. The results showed that starch granule morphology does not only vary between species of D. hispida and D. alata, but also among varieties of those species. The method used was a simple preparation of starch granules with 10% iodine staining and transparent polish to prevent the preparations from shifting. The shape of starch granules in D. hispida is dominantly polygonal (polyhedral). It showed similar results as those reported by other authors in their studies, except that-in this study-we found there is also a triangular shape, which has never been reported before. The Starch granule size of D. hispida is found to be "Very small-Small" and only one variety of the species-that is Gadung Jahe (DC9)-is classified as "Small" type. According to previous studies, varieties with small type starch granule are recommended as potential sources for non-food industrial raw materials. The shape of starch granules in D. alata is similar to those reported in other studies, which is dominantly triangular and polygonal; the size is classified as "Medium" type, except for two varieties that are Uwi Ratu (DC4) and Uwi Ulo (DC5), which are classified as "Large". According to previous studies, varieties with large type starch granule are recommended as potential sources of food. Thus, from this study three potential local varieties are identified, i.e. gadung jahe of D. hispida, and Uwi Ratu (DC4) and Uwi Ulo (DC5) of D. alata.
Bulbophyllum trinervosum (Orchidaceae) was described in mid 2020 as a new species from West Java, Indonesia. After careful examination and morphological comparisons, it has been concluded that the characters of B. trinervosum fall within the variation of widespread species, B. ovalifolium. Thus, B. trinervosum is proposed here as a synonym of B. ovalifolium. Detailed comparisons, including a series of photos of B. ovalifolium are provided.
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