Adults and larvae of the scarab beetle Frickius variolosus Germain are described. Frickius and Taurocerastes Phil. are considered sister genera. Both adult and larval characters show these to be most closely related to the geotrupine genus Ceratotrupes Jekel and they are placed in the tribe Geotrupini. A phylogeny is presented for the four tribes of the Geotrupinae, nine subfamilies of Scarabaeidae, and the Passalidae and Lucanidae based on analysis of 39 adult and larval characters.
The Canadian Entomologist 125: 1055-1 066 (1 993) An aqueous suspension of the nuclear polyhedrosis virus of Lymantria dispar (L.), LdNPV, was fed to third-instar caterpillars of L. dispar and 46 species of nontarget Lepidoptera in four successive. 24to 48-h doses of 3 x 1OQpolyhedral inclusion bodies (PIES) in 2 pL applied to small pellets of artificial diet or isolated surfaces of foliage. Adults of the fly Cyrtophlaha coqtrillerri Aldr.. and adult males of the bee Megachile rotundata (Fabr.). were assayed with a single dose of 1.2 x lo5 PIBs in 2 pL of 30% sucrose solution. Only those specimens that completely consumed the dose(s) were transferred to appropriate maintenance conditions for 7-10 days whereupon they were frozen. Samples of macerates of experimental specimens were dot-blotted onto nylon membranes on which whole genomic LmVPV DNA-probing and chemiluminescence techniques were used to show presence of LdNPV DNA. With reference to positive and negative controls, the 48 nontarget species were diagnosed as nonperrnissive orLarNPV but the target species waq clearly infected. This study demonstrates a high degree of host-specificity of LdNPV.
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