A significant increase in cardiovascular disease prevalence among the Indonesian according to Riskesdas in 2018. Therefore an alternative treatment with minimum side effects to decrease serum total cholesterol is required. Some herbal with anti-hyperlipidemic activity and has a great safety profile can be combined as a mixture to achieve high pharmacology activity with a better taste. Three types of herbal formulas were made from the combination of the juice of garlic, roselle calyx, red ginger, citrus lemon, pineapple, apple cider vinegar, and honey. The variations of the formula were based on the addition of roselle, and pineapple to the composition of garlic, red ginger, citrus lemon, apple cider vinegar, and honey. The doses used in each formula were 2.7 ml/kg BW and 5.4 ml/kg BW. Each formula was given once a day for 10 th day along with high lipid diet-PTU to the animals. Total cholesterol levels were measured digitally through tail vein one day before and after treatment. All herbal juice formula significantly decreased of total cholesterol level of the high lipid diet-PTU rat and it's linear with the dose. The addition of roselle calyx and pineapple each on the herbal juice formula will raise the anti-hyperlipidemic effect. The herbal juice contained roselle showed the highest antihyperlidemic effect at the dose of 5.4 ml/kg BW.