The main objective of this research was to study the diversity of maize inbred lines based on phenotypic characters and SSR markers. The research, which was conducted from February to June 2011, was divided into field and laboratory research for molecular analysis. The molecular analysis was conducted at the Biotecnology Laboratory, Faculty of Agriculture, Brawijaya University Malang. The field trial was done in Kandat, Junrejo, Batu. Materials of the research were 35 genotypes of maize. Steps for molecular analysis included: (1) DNA isolation, (2) DNAquality test, (3) PCR SSR, and (4) Visualization of the amplification result. The field trial was done by planting genotypes using a RCBD with 2 replications. Result of the dendogram analysis showed that 35 genotypes of maize were divided into 2 main clusters, A and B, that having 35% similarity level. Result for the analysis of variance showed significant difference among genotypes of maize on the entire of the observed phenotype characters. Heritability (H) ranged from 61.8% to 98.1%. Coefficient of variance ranged from 0.71 to 7.20%. Analysis on the genotypic correlation ranged from 0.06 to 1.0. Numbers of rows per ear showed significant correlation, in which Jaccard's similarity coefficient (r s ) = +0.43.
Abstract. Kustanto H. 2023. The phenotypic and genetic diversity test of several inbred lines on the 7th generation of melon (Cucumis melo). Biodiversitas 24: 2623-2629. The needs for melon (Cucumis melo L.) keep increasing year by year. Some efforts to increase the quality of melon have been carried out to obtain better quality and quantity through plant breeding programs. Providing new melon inbred lines with different phenotypic and genotypic traits is very important in assembling melon hybrid varieties with new genetic characteristics, potentially production superior melon varieties. The study’s objective was to find out the genetic diversity of the tested melon genotypes and to obtain inbred lines of melon, which are the potential to be developed as superior hybrid varieties. The inbred lines of melons showed that the diversity coefficient values of morphological traits ranged from 0.57 to 1.0. There are 3 Groups, namely Group I, II, and III. Group I comprises two genotypes: MJ 34 and HHX 015. Group II is divided into 2, Group A and B. Gaboup A comprises three genotypes: MJ 25, Amanda variety as the standard of comparison, and MO 29, while Group B has one genotype: HHA 02. Group III comprises three genotypes: MSO 12, MSP 13, and HHAL 01. The traits are as follows: leaf length, leaf width, fruit length, fruit diameter, and the fruit flesh thickness have closely related to yield per plant.
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