This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of Muntingia calabura L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as Escherichia coli, Salmonella typhi and Propionebacterium acnes bacteria. The variations in the concentration of the ethanol extract of M. calabura included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the M. calabura leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the M. calabura leaves against E. coli (14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52), S. typhi (13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and P. acnes (14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against E. coli, S. typhi and P. acnes are 31.25±2.08; 25.15±1.61; and 23.25±4.42.

Ketumbar (Coriandrum sativum L.) is a plant that is widely used as a spice, especially in cooking spices. This plant has a distinctive aroma. The distinctive aroma that is owned because this plant contains essential oils. 1 Phytochemical screening results of coriander seeds have diverse secondary metabolites, including steroids, flavonoids, saponins, tannins, coumarin, 2 volatile compounds 3 and coriander leaves are contain phenolic acid, poliyphenols, glycosides, saponins, flavonoids and tannins. 4,5 These coriander plants have diverse biology activities including antioxidant, 6 antimicrobial, hypoglycemic, hypolipidemic, anxiolytic, analgesic, anti-inflammatory, anti-convulsant 3 and anti-cancer activities and gastrointestinal, antiinflammatory, antiseptic, tranquilizing nervous system, lipolytic and miorelaksan, rerigeran, tonic, dieretic, rheumatic, neuralgia, and flatulence 7 and antimicrobials. 8 This study aims to characterize, isolate essential oils and analyze the content of bioactive compounds of C. sativum leaves using Gas Chromatography-Mass Spectrocopy (GC-MS). MATERIALS AND METHODS Preparation sampleFresh of ketumbar (C. sativum) leaves were obtained from the Berastagi area, Karo district, North Sumatera, Indonesia. The C. sativum leaves is authorized by the Indonesian scientific institution: Biology Research Center (3533/MEDA/2019). Samples are cleaned in running water, drained, and dried in open spaces which avoid direct contact with sunlight. The dried sample was mashed using a blender to obtain the simplicia powder of C. sativum leaves. Preparation of isolation essential oil of C. sativum leavesIsolation of essential oils of simplicia of C. sativum leaves was carried out by means of modified distillation (Figure 1). Simplisia C. sativum leaves are put into a round pumpkin, plus boiling stones and distilled water to taste. The distillation process is carried out for 1-2 days per 500 g simplicia of C.