ABSTRAKZea mays L. (Jagung) adalah tanaman yang banyak dibudidayakan di Indonesia dan pemanfaatan limbah dari jagung masih sebatas sebagai pakan ternak, sedangkan pemanfaatan kandungan komponen didalamnya masih sangat terbatas. Kandungan kimia yang terdapat pada tanaman jagung antara lain alkaloid, saponin, tanin, flavonoid, fenol, steroid, glikosida, terpenoid, protein, mineral. Senyawa fenol banyak berperan dalam aktivitas antioksidan dan tabir surya. Sampel tongkol dan rambut jagung diduga berpotensi sebagai sumber antioksidan dan tabir surya alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan tabir surya dari ekstrak dan fraksi tongkol dan rambut jagung serta kadar fenol totalnya. Uji aktivitas antioksidan dilakukan dengan menggunakan metode peredaman radikal bebas 1,1 Difenil-1-Pikrihidrazil (DPPH) dan uji aktivitas tabir surya dengan penentuan nilai sun protected farctor (SPF) dengan spektrofotometri yang diukur pada λ 290-320nm. Penetapan kadar fenol total diukur dengan metode kolorimetri menggunakan reagen Folin Ciocalteu dan diukur dengan spektrofotometri pada λ 765nm. Hasil pengujian aktivitas antioksidan dan tabir surya terbaik ditunjukkan oleh fraksi etil asetat rambut jagung dengan nilai IC 50 sebesar 45,18 μg/mLdan nilai SPF sebesar 23,943 serta kadar fenol totalnya sebesar 106,010 mg/G ± 0,431. Fraksi etil asetat rambut jagung memiliki aktivitas antioksidan, tabir surya, dan kadar fenol yang lebih tinggi dari yang lainnya.Kata Kunci : Tongkol dan rambut jagung (Zea mays L.), kadar fenol, antioksidan dan tabir surya. Antioxidant and Sunscreen Activities of Corn Cob and Corn Silk of Zea maysABSTRACT Zea mays L (corn) is a kind of plantation widely cultivated in Indonesia. The corn waste is often used as cattle feed, while the component inside it is still limited. The chemical components existing in the corn are alkaloid, saponin, tannin, flavonoid, phenol, steroid, glicoside, terpenoid, protein, and mineral. The phenolic compounds mostly takes part in the activities of antioxidant and sunscreen. The sample of corn cob and corn silk is considered to be potential as the source of natural antioxidant and sunscreen. This research was conducted to determine the activities of antioxidant and sunscreen of the extract and fractions of corn cob and corn silk, and also the total phenolic compounds. The whole extracts and fractions were then tested their antioxidant and sunscreen activity and total phenolic content. The antioxidant activity was carried out by 1.1 of Difenil-1-Pikrihidrazil (DPPH) method and the sunscreen activity by determination the value of Sun Protected Factor (SPF) by spectrofotometry method. The determination of the total phenolic compound was analysed using the Folin Ciocalteu reagent. The best antioxidant and sunscreen activities showed on ethyl acetate fractions of cornsilk where as IC 50 45.18 μg/mL and SPF 23.943. Total phenolic compound of ethyl acetate fractions of corn silk was 106.010 mg/G ± 0.431. Ethyl acetate fractions of corn silk has antioxidant activi...

Etlingera elatior traditionally used for therapeutic purposes. Pharmacological activities of this plant have been reported, one of which is cytotoxic activity. This study aims to isolate cytotoxic compounds against HeLa cervical cancer cell lines from an ethanol extract of the E. elatior leaves. The isolation work was conducted by means of column chromatography and determination of the molecular structure was by spectroscopic data analysis. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The results of this study were two isolated compounds identified as quercetin and quercetin-3-O-rhamnoside from the ethyl acetate fraction. These two compounds had cytotoxic activity on HeLa cervical cancer cells which was indicated by the inhibition of cell proliferation with IC 50 values were 29.49 and 46.67 µg/mL, respectively. In the migration assay, quercetin and quercetin-3-O-rhamnoside inhibited migration of the HeLa cells significantly at 10 µg/mL as compared with the control, in 24 and 48 hr experiments. This study reinforces the previous evidence of the potential of quercetin and quercetin-3-O-rhamnoside as an anticancer drug candidate.

AbstrakPengembangan obat dan kosmetik dari biota laut kini tengah terjadi di dunia farmasi. Mikroalga Thalassiosira sp merupakan jenis mikroalga yang memiliki kandungan senyawa-senyawa bioaktif. Beberapa penelitian sebelumnya menunjukkan bawa mikroalga memiliki aktivitas antibakteri.  Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak mikroalga Thalassiosira sp  terhadap 3 bakteri  yaitu staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne di fasa n-heksan, etil asetat dan etanol. Ketiga bakteri ini dapat menyebabkan infeksi kulit. Mikroalga Thalassiosira sp dikultivasi menggunakan medium walne dan di panen pada hari ke-6 setelah penanaman. Pemanen mikroalga dilakukan dengan teknik sentrifuga. Ektrak dilakukan dengan cara  maserasi bertingkat selama 3 x 24 jam. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstrak mikroalga Thalassiosira sp diperoleh paling banyak pada ekstrak etanol sebanyak 24,24%(b/b), ektrak etil asetat sebanyak 19,75%(b/b) dan paling sedikit adalah ekstrak heksan sebanyak 8,64% (b/b). Hasil uji difusi menunjukkan ekstrak n-heksan, etil asetat dan etanol mikroalga Thalassiosira sp memiliki aktivitas terhadap bakteri staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne yang ditunjukan dengan adanya zona bening. Kata kunci: mikroalga, Thalassiosira sp, antibakteri, infeksi kulit, metode difusi Abstract Development drugs and cosmetics from marine biota is now being happened in pharmacy word. Microalgae Thalassiosira sp is a type of microalgae that has a bioactive compounds. Several previous studies have shown the existence of microalgae which have antibacterial activity. This study aimed to study the antibacterial activity of extracts of microalgae Thalassiosira sp against 3 bacteria which is staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne in the n-hexane, ethyl acetate and ethanol phases. These three bacteria can cause skin infections. Microalgae Thalassiosira sp was cultivated using walne medium and harvested on the 7th day after planting. Microalgae harvesters are carried out by centrifuge techniques. The extract is done by multilevel maceration for 3 x 24 hours. Antibacterial testing was carried out by the paper diffusion method or Kirby-Bauer's disc diffusion method. The results of the crude extract of microalgae Thalassiosira sp were obtained at most in ethanol extract as much as 24.24% (w/w), extract of ethyl acetate at 19.75% (w / w) and at least hexane extract at 8.64% (w/w). The diffusion test results for n-hexane, ethyl acetate, and ethanol microalgae Thalassiosira sp extract have activity on  staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne which are supported by clear zones. Keywords: mikroalge, Thalassiosira sp, antibacteria, skin infection, diffusion method