Sesame (Sesamum indicum L.) is the most important oil seed crop that cultivated widely in many regions where several environmental conditions, agronomical practices, postharvest processes, and oil extraction approaches significantly influence the quality of sesame seeds oil. The combined effect of roasting treatment and growing conditions on sesame seeds and oil was scarcely studied, therefore, this study investigated the impact of microwave roasting on the physicochemical characteristics and bioactive properties of sesame seeds and oil. Microwave roasting treatment increased the antioxidant activity, total phenolic, oil content, p-anisidine and peroxide values, and tocopherol content of sesame seed samples to different enhancement magnitudes depending on sesame growing country and microwave roasting conditions. The treatment also increased L*, a*, and b* values and reduced the moisture content of sesame seeds compared to controls. The main fatty acids in oils extracted from sesame seeds obtained from different countries were oleic and linoleic acids. 2.2.2 | Color analysis of sesame seeds Color values were measured using a Minolta Chroma meter CR 400 (Konica Minolta, Inc. Osaka, Japan). The device was calibrated against the white surface calibration plate before measurement and the L*, a*, b*, and ΔE* values were determined according to the CIELab color scale. 2.2.3 | Determination of moisture, ash, protein, and oil The amounts of moisture, ash, protein, and oil in sesame seeds were measured according to AOAC method (2010). 2.2.4 | Extraction of bioactive compounds Bioactive compounds were extracted from raw and roasted sesame seeds as described previously (Jannat et al., 2010). Briefly, ground sesame seed samples (2.5 g) were extracted in 10 ml of aqueous How to cite this article: Mohamed Ahmed IA, Musa Özcan M, Uslu N, et al. Effect of microwave roasting on color, total phenol, antioxidant activity, fatty acid composition, tocopherol, and chemical composition of sesame seed and oils obtained from different countries.