Background : The physiological regulation of the ovary is a complicated process ; the growth factor, steroid hormones, gonadotropins, and cytokines all take part. Inflammatory cytokines are found in many compartments of the ovary, such as the follicles. The ovarian tissue produces them in addition to the macrophages. In this study, we aimed to compare the level of serum and follicular fluid IL-1Beta (IL-1β β) for different groups of infertile patients, comparing the ICSI outcome between sub-fertile couples due to different causes. The results of this study IL-1β β was significantly higher in the patients with unexplained subfertility (p-value < 0.05), no significant difference was found in the ICSI outcome between patients with different causes of subfertility. The lowest pregnancy rate was found in patients with unexplained subfertility, but the difference is insignificant. In conclusion, immunity plays a role in unexplained subfertility as patients with unexplained subfertility have a significantly higher serum level IL-1β β than the other causes of subfertility. However, IL-1β β does not affect ICSI outcome. Therefore, the ICSI outcome is not affected by the cause of subfertility.

Subfertility is a universal, general problem of male and females factor. Zinc is one of the most important trace mineral, that plays an essential and necessary role in sperm generation and normal, physiological male reproduction. Pituitary Gonadotropins (FSH) and (LH) are basic for reproduction, FSH is a crucial role for sperm production and LH energize synthesis and production of testosterone Hormone. Currently, the most common therapy used in case of male subfertility clomiphene citrate, the study show significant increase in semen parameter and hormonal status after treatment with zinc 50 mg once/day and clomiphene citrate 50 mg twice/day for at least 2- 3 months. The aim of this research is. To evaluate the role of the zinc and clomiphene citrate on male subfertility case.