Nanoparticle application in microalgae for enhanced lipid production is an ongoing work that leads towards the contribution in biodiesel production. During this decade, metal nanoparticles are constantly being reported to have numerous applications in diverse fields, because of their unique optical, electrical, and magnetic properties. They can interact with the biomolecules of cells and thereby alters cellular metabolisms, which in turn reflects their ability to regulate some primary or secondary metabolic pathways. Nanoparticles derived from metals like Fe, Cu, and Se are taking part in redox processes and their presence in many enzymes may modulate algal metabolisms. Besides by upregulating or downregulating the expression of several genes, nanoparticle exposure can alter gene expressions in many organisms. In microalgae such as Chlorella vulgaris, C. pyrenoidosa, Scenedesmus obliquus, S. rubescens, Trachydiscus minut u s, Parachlorella kessleri , and Tetraselmis suecica ; metal nanoparticle exposure in different environmental conditions have impacts on various physiological or molecular changes, thereby increasing the growth rate, biomass and lipid production. The present mini-review gives an insight into the various advantages and a future outlook on the application of nanoparticles in microalgae for biofuel production. Also, it can be proposed that nanoparticles could be useful in blocking or deactivating the AGPase enzyme (involved in the glucose to starch conversion pathway), binding to its active site, thereby increasing lipid production in microalgae that could be utilized for enhanced biodiesel production.

Here we respond to the paper entitled “Contribution of anthocyanin pathways to fruit flesh coloration in pitayas” (Fan et al., BMC Plant Biol 20:361, 2020). In this paper Fan et al. 2020 propose that the anthocyanins can be detected in the betalain-pigmented genus Hylocereus, and suggest they are responsible for the colouration of the fruit flesh. We are open to the idea that, given the evolutionary maintenance of fully functional anthocyanin synthesis genes in betalain-pigmented species, anthocyanin pigmentation might co-occur with betalain pigments, as yet undetected, in some species. However, in absence of the LC-MS/MS spectra and co-elution/fragmentation of the authentic standard comparison, the findings of Fan et al. 2020 are not credible. Furthermore, our close examination of the paper, and re-analysis of datasets that have been made available, indicate numerous additional problems. Namely, the failure to detect betalains in an untargeted metabolite analysis, accumulation of reported anthocyanins that does not correlate with the colour of the fruit, absence of key anthocyanin synthesis genes from qPCR data, likely mis-identification of key anthocyanin genes, unreproducible patterns of correlated RNAseq data, lack of gene expression correlation with pigmentation accumulation, and putative transcription factors that are weak candidates for transcriptional up-regulation of the anthocyanin pathway.

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