No abstract

As Japan continues to become a popular global tourism destination, its vulnerability to disasters cannot be overlooked. Although the Japanese government has implemented different disaster prevention policies and practices, there is a lack of investigation into disaster response behaviors, such as information seeking, evacuation, and the factors affecting these. This study clarified the factors influencing the disaster response behavior of international tourists in the context of the anticipated Tokyo Metropolitan Earthquake scenario. A questionnaire survey was conducted, and the logistic regression model was used to understand the causative factors. The results revealed that the variable knowledge on disaster response behavior in earthquakes influences individuals' evacuation decisions, especially when people choose to follow others. These findings will be beneficial for tourism stakeholders and policymakers to establish effective tourism crisis management planning, including information provision, shelter allocation, and collaborative initiatives.

No abstract

Eukaryotic precursor tRNAs (pre-tRNAs) often have an intron between positions 37 and 38 of the anticodon loop. However, atypical introns are found in some eukaryotes and archaea. In an early-diverged red alga Cyanidioschyzon merolae, the tRNAIle(UAU) gene contains three intron coding regions, located in the D-, anticodon, and T-arms. In this study, we focused on the relationship between the intron removal and formation of pseudouridine (Ψ), one of the most universally modified nucleosides. It had been reported that yeast Pus1 is a multiple-site-specific enzyme that synthesizes Ψ34 and Ψ36 in tRNAIle(UAU) in an intron-dependent manner. Unexpectedly, our biochemical experiments showed that the C. merolae ortholog of Pus1 pseudouridylated an intronless tRNAIle(UAU) and that the modification position was determined to be 55 which is the target of Pus4 but not Pus1 in yeast. Furthermore, unlike yeast Pus1, cmPus1 mediates Ψ modification at positions 34, 36, and/or 55 only in some specific intron-containing pre-tRNAIle(UAU) variants. cmPus4 was confirmed to be a single-site-specific enzyme that only converts U55 to Ψ, in a similar manner to yeast Pus4. cmPus4 did not catalyze the pseudouridine formation in pre-tRNAs containing an intron in the T-arm.