This research is motivated by the low achievement of sciencem literacy of Indonesian students in the participation of the study of The Programe for International Student Assessment (PISA) held every three years by The Organization for Economic Co-operation and Development (OECD). This study aims to determine the quality of science literacy profile of junior high school students. The sample amounted to 356 students of SMP class IX, taken using proportionate stativeied random sampling technique. The Method is test and interview. The tests given using questions obtained from the OECD published PISA issue were published in 2009 specifically for matters related to science content. The results showed that the Literacy Quality of Science Profile of Junior High School Students in Pati Regency belong to low category with percentage of 55%. In the category of moderate percentage obtained by 45% and no students who fall into the high category. As for the achievement of the value of each level, the highest ability of students in answering science literacy questions in the level 1 questions with a total of 49.43 moderate categorized, and the lowest ability of students in answering the questions of science literacy is in level questions 6th with a total of 13.48 is low categorized. The conclusion is the quality of science literacy profile of junior high school students in Pati District is low.