This study aims to determine the effectiveness of supplementation of sodium (Na) alginate Sargassum sp. from Lampung waters to enhance the non-specific immune response and the resistence of Pacific white shrimp (Litopenaeus vannamei) against white spot disease. This study used a completely randomized design with 3 treatments, namely feeding without alginate supplementation (A) or control, dietary Na alginate Sargassum supplementation at a dose of 2.0 (B), and 4.0 g kg−1 feed (C), each with four replications, for 14 days. Hemolymph sampling was performed on days 0, 7 and 14 to observe the parameter of shrimp hematology included total haemocyte count (THC), phagocytocyte activity (PA), phagocytic index (PI), and total plasma protein (TPP). The hepatopancreas histology profile and water quality were observed at the end of treatment. Data were analyzed by Anova at 95% of confidence interval and continued with Duncan test. Result showedthat the supplementation of Na alginat from Sargassum sp. significantly enhance several shrimp immune response namely THC, PA, and TPP. Meanwhile, the histology of the hepatopancreas of both shrimp treatment and control showed not significantly effect on tissue damage. The water quality was still in normal condition during the treatment. The results of this study indicate that the application of Sargassum alginate supplementation at a dose of 2gr/kg of feed is the best treatment to enhance the immune response of Pacific white shrimp.

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.

Usaha budidaya ikan menunjukkan perkembangan yang pesat dari tahun ketahun. Hal ini dikarenakan semakin bertambahnya kesadaran masyarakat untuk mengkonsumsi ikan. Salah satu usaha budidaya yang banyak dilakukan masayarakat indunesia adalah budidaya ikan konsumsi air tawar. Teknologi budidaya ikan konsumsi air tawar yang saat ini banyak digunakan di Indonesia adalah sistem budi daya intensif dengan padat tebar yang tinggi. Sama seperti usaha budidaya perikanan lainnya, masalah utama dalam budidaya ikan air tawar adalah serangan penyakit. Untuk menghindari keadaan ini, perlu dilakukan upaya pencegahan dan penanggulangan penyakit secara tepat. Pencegahan penyakit ikan dapat dilakukan dengan cara peningkatan daya tahan tubuh ikan dengan pemberian imunostimulan alami. Imunostimulan alami yang mudah didapat di lingkungan sekitar antara lain kunyit, bawang putih, meniran, dan masih banyak lagi. Pencegahan penyakit juga dapat dilakukan dengan cara mengontrol kualitas air, baik dari suhu, kandungan oksigen terlarut, derajat keasaman, dan mengontrol jumlah bakteri pathogen dalam perairan. Kontrol jumlah bakteri dalam perairan biasanya dilakukan dengan pemberian probiotik. Sedangkan untuk pengobatan penyakit ikan dapat dilakukan dengan cara karantina ikan atau memisahkan ikan yang sakit dengan yang sehat dan juga pemberian obat-obatan alami. Aplikasi pemberian probiotik dan imunostimulan pada media budidaya dan pakan ikan lele mampu mencegah dan mengobati penyakit pada ikan lele sehingga mampu meningkatkan produksi ikan lele. Kendala yang dihadapi dalam budidaya ikan lele di Pokdakan Ulam Adi Jaya adalah kurangnya sarana dan prasarana budidaya terutama aerator.

Pokdakan Maju Bersama Mesuji, which is in Sungai Badak Village, Mesuji District, Mesuji District, Lampung, is a group of economically productive people and potential entrepreneurs with fish farming activities in the form of growing consumption fish and local fish, especially snakehead fish. The difficult problems in the development of snakehead culture in Sungai Badak village, Mesuji Regency, are the limited availability of seeds and the slow growth of fish. These cause the business to be carried out on-off or suffer losses. This community service aims to increase the knowledge and skills of pokdakan members in producing continuous and quality snakehead seeds independently and stimulate job creation. This activity was carried out through Focus Group Discussions, and organizing counseling and training on snakehead fish hatchery technology and increasing the growth of snakehead fish with the application of recombinant growth hormone. Participants were given a written test to measure how successful the technology transfer is. The results obtained from this activity were that the knowledge and skills of the trainees increased from an average score of 58 to 85 in hatchery technology transfer (32%) and increased from an average score of 35 to 66 in the transfer of recombinant growth hormone application technology (31%) (scale 0- 100). The increase in the value of these participants is presumably because most of the participants are fish cultivators who are used to carrying out aquaculture activities but do not know the basics. So that with this activity it is hoped that it can improve the economy of cultivator families and make Mesuji Regency a fish center and support inclusive and sustainable economic growth. In general, the two technology transfers in this activity increased the percentage of participants' knowledge and skills by 31.5% and this activity was successful.

This study aims to analyze the effect of the various concentration of guava leaf extract (Psidium guajava Linnaeus) on the prevention of Motile Aeromonas Septicemia disease in common carp (Cyprinus carpio Linnaeus). In a total of 150 common carp, length 9-11 cm with a density of 10 carps/aquarium. This study used a completely randomized design through 5 treatments, each treatment has 3 replications, namely (K+) consists of without guava Psidium guajava leaf extracts and without bacteria-infection Aeromonas hydrophila, (K-) consists of without guava Psidium guajava leaf extracts with bacteria-infection Aeromonas hydrophila, (A) the application of 125 ppm guava Psidium guajava leaf extracts, (B) the application of 250 ppm guava leaf extracts, (C) the application of 500 ppm guava Psidium guajava leaf extracts for 23 days. The parameters observed total of leukocytes, differensial leukocytes, hematocrit levels, erythrocyte, hemoglobin, phagocytic activity (AF), phagocytic index (IF), relative percent survival (RPS), survival rate (SR) and water quality. Data were analyzed with Anova 95% confidence interval and continued with Duncan test. The parameter also showed a significant result (P<0,05) with the application of guava Psidium guajava leaf extracts to the total leukocytes, differensial leukocytes, erythrocyte, hemoglobin, phagocytic activity, phagocytic index and relative percent survival. The results of this research indicate the concentration of 250 ppm guava Psidium guajava leaf extracts is the optimum application to increase common carp non-spesific immune responses.