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Plant tissue culture method has an impressive technique for Investigation and Explains basic and applied problems in plant biotechnology field. Micropropagation has played a vital role in the rapid multiplication of many plants species. The nodal explants and shoot tip of N. jatamansi inoculated in MS medium (Murashige and Skoog) contain different types concentrations of PGRs (Phytohormones) at various frequencies for the optimization of growth quality for shoot bud Induction, shoot proliferation and micro rooting in plant. The perfect shoot induction takes place in the concentration of BAP + IBA (2.0 mg/l +1.5 mg/1) multiplication of nodal explants and shoot tip in the combination lower concentration of BAP and KN (2.0 mg/1+1.5 mg/1) This combination proved best for multiple shoot formation. Half strength (1/2) of the MS medium containing NAA and BAP (1.5 mg/1+1.0 mg/1) in combination was most useful for rooting in plant. Well developed rooted micro shoots were smoothly removed for the culture flask and dipped in 70% ethanol for 2 min and then washed with running tap water for 5-10 min to remove media for the root and transferred to small plastic cups carry cocopeat, garden soil and sand (2:2:1) and produce healthy growth in ex-vitro conditions.

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Dyes play a very important role in our daily lives. The dye industries started manufacturingdyes with natural sources and then turned towards synthetic ones. Among these dyes, about60% of the total dyes are azo dyes which are used in industries. They are mostly used as textiledyes because they are easy to synthesize, chemically stable and diverse in nature. Azo dyescome in about 3000 different variations and are employed in a variety of industries, includingthe textile, leather, paper, and pharmaceutical sectors. But sadly, the majority of azo dyes arepoisonous and mutagenic to all living things. The effluent coming from the textile sector musttherefore be removed and treated. The wastewater is often treated using a variety of physicaland chemical methods, however, these methods have been shown to be inefficient, expensive,produce insufficient amounts of sludge, and have limited effectiveness. So, in order to treatand decolorize dyes and dye-containing effluents without further harming the environment orendangering life forms, It has practically universal dye degradability is economical and hasalso eliminated a number of drawbacks of the physicochemical method. The properties andclassification of azo dyes, associated problems, biodegradation techniques, and possibilitiesare only a few of the subjects covered in this chapter’s examination of recent research,advancements, and the body of existing information on them. Biological processes havereceived special attention as a remedy for the current problems with azo dyes.