Segmental selective adrenal venous sampling (sAVS) elucidates an intraadrenal aldosterone activity map (IAMap), which allows us to design a novel surgical treatment strategy for patients with primary aldosteronism. We evaluated the usefulness of sAVS by analyzing 278 patients with whom we had prospectively used IAMap using the criteria of sAVS for surgical indication between 2009 and 2015. We evaluated its diagnostic accuracy using pathological and postsurgical biochemical and clinical outcomes. One hundred twenty and 158 patients were diagnosed with unilateral and bilateral disease, respectively, through sAVS. The concordance of lateralization diagnosis with computed tomography imaging was 66.6%. Among the unilateral patients, we performed partial adrenalectomy in 68 patients whose IAMap showed focal aldosterone hypersecretion from computed tomography-detectable tumor in the affected adrenal gland. All of them achieved complete biochemical success 1 year after surgery. Furthermore, 25 of 158 bilateral disease patients underwent surgical resection because they were preoperatively diagnosed as bilateral aldosterone-producing adenomas by IAMap. These cases showed complete or partial biochemical success (28.0% and 72.0%, respectively); 36.0% showed complete clinical success. Pathological studies demonstrated that all 145 resected specimens possessed aldosterone-producing adenoma or multiple nodules (132 and 13 cases, respectively), and none showed diffuse hyperplasia. IAMap accurately diagnosed both bilateral and unilateral aldosterone-producing adenomas and diffuse hyperplasia before surgery. sAVS allows a novel surgical strategy for selected PA patients with favorable outcomes.

No abstract

Protein prenylation is essential for many cellular processes including signal transduction, cytoskeletal reorganization, and membrane trafficking. Here, we identify a novel type of protein prenyltransferase, which we named geranylgeranyltransferase type-III (GGTase-III). GGTase-III consists of prenyltransferase alpha subunit repeat containing 1 (PTAR1) and the b subunit of RabGG-Tase. Using a biotinylated geranylgeranyl analogue, we identified the Golgi SNARE protein Ykt6 as a substrate of GGTase-III. GGTase-III transfers a geranylgeranyl group to mono-farnesylated Ykt6, generating doubly prenylated Ykt6. The crystal structure of GGTase-III in complex with Ykt6 provides structural basis for Ykt6 double prenylation. In GGTase-III-deficient cells, Ykt6 remained in a singly prenylated form, and the Golgi SNARE complex assembly was severely impaired. Consequently, the Golgi apparatus was structurally disorganized, and intra-Golgi protein trafficking was delayed. Our findings reveal a fourth type of protein prenyltransferase that generates geranylgeranyl-farnesyl Ykt6. Double prenylation of Ykt6 is essential for the structural and functional organization of the Golgi apparatus.

SummaryThe demand for biofuels has recently increased because of rising prices of fossil fuels and diversification of energy resources. As a result, the demand for sugarcane and corn has been increasing, not only for food production, but also as sources of energy. In this context, securing supplies of phosphorus, required as an essential nutrient in agricultural production, has considerable implications that extend beyond food and agricultural policy. It is therefore important to consider the quantity and availability of phosphorus resources that remain untapped, because the demand and supply of phosphate ore is currently becoming very tight, and Japan has no domestic phosphorus resources.To identify potential phosphorus resources, we have investigated the material flow of phosphorus within Japan, including that in the iron and steel industry, on the basis of statistical data for 2002. Our major finding is that the quantity of phosphorus in iron and steelmaking slag is almost equivalent to that in imported phosphate ore in terms of both the amount and concentration. We also found, by means of a waste inputoutput analysis and a total materials requirement study, that the phosphorus potentially recoverable from steelmaking slag by a new process that we have proposed has considerable environmental and economic benefits. Concerning the restricted supplies of phosphorus resource, it is important to consider the quantity and availability of phosphorus resources that currently remain untapped. From that viewpoint, steelmaking slag would be expected to be a great potential resource for phosphorus.

Table 5. The environmental and economic effects of recovery of phosphorus from steelmaking slag, estimated by the waste input-output model.