Aim: Nosocomial infection (NI), or so-called hospital-acquired infection, is one of the most important problems facing us and the world at this time. This study aims to investigate the effect of minimum inhibition concentration of Ag-NPs on isolated bacteria from nosocomial infections. Place and Duration of Study: Gastroenterology Surgical Center, Mansoura University, Mansoura, Egypt, between July 2017 and July 2018. Methodology: A total of 368 different samples of 100 patients were taken which staying for 3 days or more in Gastroenterology Surgical Center, Mansoura University, Egypt. Three different types of Ag-NPs ‎synthesized by three different chemical methods were used. Results: The results showed that the highest rate of infection of NI was found in K. Pneumoniae with an estimated percentage of 40%. The size of spherical Citrated-Ag-NPs ‎‎ was found in the range of 15-57 nm. On the contrary, the size of spherical Polyvinyl-Pyrrolidone (PVP) and PVP Glucose Ag-NPs were found to be smaller with size ranging from 7.8-23 and 7.58-25 nm, respectively.  Conclusion: Our findings showed that PVP Glucose Ag-NPs had the highest impact on all types of isolated bacteria associated with nosocomial infections.

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Aims: Nosocomial infection (NI) is one of the most important problems facing the world. This work is dedicated to investigating the prevalence rates of NI in addition to estimating the minimum inhibition concentration (MIC) of commercial antibiotics on isolated bacteria in order to determine the most diluted antibiotic that performed well with more efficiency and minimal toxicity. Place and Duration of Study: Gastroenterology Surgical Center, Mansoura University, Mansoura, Egypt, between July 2017 and July 2018. Methodology: This study included 368 different samples (urine, stool, sputum and surgical wounds) from 100 patients A total of 15 commercial antibiotics selected from seven families having different mode of action were used. Results: Our findings demonstrated that the highest prevalence rate of NI was detected in K. pneumoniae (40%, n=56) followed by MRSA (22.85%, n=32), E. coli (20%, n=28), P. mirabilis (7.85%, n=11), P. putida (5%, n=7) and finally P. aeruginosa (4.3%, n=6). In addition, the results showed variable MICs of various antibiotics on isolated bacteria associated with NI. Conclusion: Interestingly, our findings showed that quinolones family had the highest impact on all types of isolated bacteria associated with nosocomial infections.