Nine different food cans, packed with water, were heat-treated for 30 min at 80 or 100°C in order to elucidate the trends associated with the leaching of bisphenol A (BPA) from internal coatings. Low levels of BPA were detected in water from all unheated cans, rising to 0.06 to 32 ng/cm 2 after heating at 100°C. Reducing the heat-treatment temperature to 80°C was found to reduce the BPA concentration in the contained water by up to two-thirds. The cans that recorded the highest BPA concentrations in water after heating were found to have components (lid, bottom or body) with high available BPA contents.
We observed and evaluated the feeding behavior of the free-living nematode Caenorhabditis elegans (C. elegans) after exposure to bisphenol A (BPA) and nonylphenol (NP). Exposed organisms were transferred to chemical-free culture medium and their attainment levels (the number of worms reaching the food source divided by the total number of worms on the Petri plate) were recorded after 2, 4, 6, 8, and 24 hr. Results showed a significant decrease in the attainment level of worms exposed to 10 µM and 0.1 µM BPA. However, there was a slight increase in the attainment level of nematodes treated with 1 µM NP. These results differ from previous studies showing NP as being more lethal to nematodes than BPA.
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