Pericytes are the major source of scar-producing myofibroblasts following kidney injury; however, the mechanisms of this transition are unclear. To clarify this, we examined Collagen 1 (α1)-green fluorescent protein (GFP) reporter mice (pericytes and myofibroblasts express GFP) following ureteral obstruction or ischemia-reperfusion injury and focused on the role of platelet-derived growth factor (PDGF)-receptor (PDGFR) signaling in these two different injury models. Pericyte proliferation was noted after injury with reactivation of α-smooth muscle actin expression, a marker of the myofibroblast phenotype. PDGF expression increased in injured tubules, endothelium, and macrophages after injury, whereas PDGFR subunits α and β were expressed exclusively in interstitial GFP-labeled pericytes and myofibroblasts. When PDGFRα or PDGFRβ activation was inhibited by receptor-specific antibody following injury, proliferation and differentiation of pericytes decreased. The antibodies also blunted the injury-induced transcription of PDGF, transforming growth factor β1, and chemokine CCL2. They also reduced macrophage infiltration and fibrosis. Imatinib, a PDGFR tyrosine kinase inhibitor, attenuated pericyte proliferation and kidney fibrosis in both fibrogenic models. Thus, PDGFR signaling is involved in pericyte activation, proliferation, and differentiation into myofibroblasts during progressive kidney injury. Hence, pericytes may be a novel target to prevent kidney fibrosis by means of PDGFR signaling blockade.

No abstract

The northern part of the South China Sea is characterized by widespread occurrence of bottom simulating reflectors indicating the presence of marine gas hydrate. Because the area covers both a tectonically inactive passive margin and the termination of a subduction zone, the influence of tectonism on the dynamics of gas hydrate systems can be studied in this region. Geophysical data show that there are multiple thrust faults on the active margin while much fewer and smaller faults exist in the passive margin. This tectonic difference matches with a difference in the geophysical characteristics of the gas hydrate systems. High hydrate saturation derived from ocean bottom seismometer data and controlled source electromagnetic data and conspicuous high‐amplitude reflections in P‐Cable 3‐D seismic data above the bottom simulating reflector are found in the anticlinal ridges of the active margin. In contrast, all geophysical evidence for the passive margin points to normal to low hydrate saturations. Geochemical analyses of gas samples collected at seep sites on the active margin show methane with heavy δ13C isotope composition, while gas collected at the passive margin shows light carbon isotope composition. Thus, we interpret the passive margin as a typical gas hydrate province fueled by biogenic production of methane and the active margin gas hydrate system as a system that is fueled not only by biogenic gas production but also by additional advection of thermogenic methane from the subduction system.

This study formulates a synoptic-scale eddy (SSE) kinetic energy equation by partitioning the original field into seasonal mean circulation, intraseasonal oscillation (ISO), and SSEs to examine the multiscale interactions over the western North Pacific (WNP) in autumn. In addition, the relative contribution of synopticmean and synoptic-ISO interactions to SSE kinetic energy was quantitatively estimated by further separating barotropic energy conversion (CK) into synoptic-mean barotropic energy conversion (CK S2M ) and synoptic-ISO barotropic energy conversion (CK S2ISO ) components.The development of tropical SSE in the lower troposphere is mainly attributed to CK associated with multiscale interactions. Mean cyclonic circulation in the lower troposphere consistently provides kinetic energy to SSEs (CK S2M . 0) during the ISO westerly and easterly phases. However, CK S2ISO during the ISO westerly and easterly phases differs considerably. During the ISO westerly phase, the enhanced ISO cyclonic flow converts energy to SSEs (CK S2ISO . 0). The magnitude of the downscale energy conversion from mean and ISO to SSEs is related to the strength of the SSEs. During the ISO westerly phase, a stronger SSE extracts more kinetic energy from mean and ISO circulation. This positive feedback between SSE-mean and SSE-ISO interactions causes further strengthening of SSEs during the ISO westerly phase.By contrast, upscale energy conversion from SSEs to ISO anticyclonic flow (CK S2ISO , 0) was observed during the ISO easterly phase. The weaker SSE activity during the ISO easterly phase occurred because the mean circulation provides less energy to SSEs and, at the same time, SSEs lose energy to ISO during the ISO easterly phase. The two-way interaction between the ISO and SSEs has considerable effects on the development of tropical SSEs over the WNP in autumn.