Objective:In this study, we compared the effects of 0.12% chlorhexidine (CHX) and nano zinc oxide (NZO) on the microshear bond strength of dentin with a fifth-generation adhesive after acid etching.Materials and Methods:Forty molar teeth were randomly divided into four main groups based on dentin surface treatment technique (a) control (single bond 2); (b) NZO; (c) CHX; and (d) NZO + CHX. In each group, half of the samples underwent thermocycling, with no thermocycling in the other half. Then, failure mode was evaluated under a stereomicroscope. Statistical analysis was performed using t-test, two-way ANOVA, and Chi-squared test.Results:The mean microshear bond strength of the groups without thermocycling was more than that of the groups with thermocycling, but there were no statistically significant differences between the groups with and without thermocycling in pair-wise comparisons.Conclusion:Pretreatment with NZO and CHX separately and simultaneously had no effect on the microshear bond strength of a fifth-generation adhesive.
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