This study was conducted to investigate the effect of different methods of using probiotics Bacillus subtilis and Bacillus licheniformis in soya bean-based diets on the physiology of beluga (Huso huso) (351 ± 4 g). Five experimental diets were formulated to feed beluga for 10 weeks. The results showed that there was no significant difference in weight gain between control (446 g) and 70%SBM-SPRAY2 (409 g) treatments. Long-chain polyunsaturated fatty acids in the muscle of beluga fed control (203.2 g/kg) and 70%SBM-SPRAY2 (166.4 g/kg) diet did not change statistically. Blood performance as a newly introduced factor in individuals fed dietary control (64.06) and 70%SBM-SPRAY2 (64.33) had higher value as compared with those fed dietary 70%SBM-DIET2 (61.90) (p < 0.05). Also, total protein (1.68 g/dl) and RBC (0.74 × 10 6 /mm 3) in fish fed 70%SBM were statistically lower than other treatments (p < 0.05). Lysozyme, alternative complement activity and total viable counts in beluga fed dietary probiotics were significantly higher than others. In conclusion, feeding fish with the 70%SBM-SPRAY2 diet improved fish growth and health.