Background: Gdh1 and Gdh3 are glutamate-synthesizing isofunctional NADP-GDH in S. cerevisiae. Results: Stationary phase-specific GDH3 expression and degradation of Gdh1 were responsible for the Gdh3-dependent glutamate supply and resistance to stress-induced apoptosis in stationary phase. Conclusion: Gdh3 plays a role distinct from Gdh1 by rendering cells resistant to stress and aging. Significance: This provides mechanistic insight into apoptosis and protein degradation in response to stress.

It can be concluded that the CLP-based real-time PCR assay is a more appropriate method for sensitive detection and quantification of C. burnetii than previously reported methods.