The retention behaviour of amino acids was studied in hydrophilic LC on zwitterionic stationary phases. Evaluation of the influences of acetonitrile/water content, ammonium acetate (NH4Ac) concentration and mobile phase pH values was performed. Fourteen amino acids were tested and they were all retained to varying extents, with poorer retention in high water content eluents. The linear relationship between the logarithm of retention factor and log(water content) indicated that adsorption dominated or at least was partly involved in the separation mechanism. Electrostatic and hydrophilic interactions also contributed to the retention of these amino acids under different separation conditions with various mobile phase pH values and NH4Ac concentrations. Thus, the overall retention mechanism could be explained as a combination of adsorption, electrostatic and hydrophilic interactions. The magnitude and contribution of each mechanism is dependent on the nature of the analyte and the separation conditions applied.
Based on the large geometric deformation and nonlinear of material theories, the seismic responses of a double-layer spherical lattice shells structure were studied. The results show that under multi-dimensional earthquake, the maximum displacement and axial stress, as well as plastic ratio of the structure will be obviously increased than those corresponding to the responses under single-dimensional earthquake. When long-period seismic wave was taken as the input wave, the responses of the structure were the maximum.
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