The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge, this is the first report of the complete sequence of a NLV isolate.

Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have significant subpopulation differentiation with an FST value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with dN/dS values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from analysis of molecular variance (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. Furthermore, OrMV isolates from the genera Ornithogalum, Lachenalia and Diuri tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation.

No abstract

Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have a significant subpopulation differentiation with an F ST value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with ω-values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from molecular variance of analysis (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. In BaTS analysis, OrMV isolates from the genera Ornithogalum, Lachenalia, Diuri tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation. Furthermore, age calculations suggested that the first divergence event of the OrMV isolates analyzed might take place around 1068 BC. 17 Abstract 18 Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety 19 of ornamentals. In this study, the coat protein (CP) gene of OrMV was used to investigate the 20 molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two 21 groups which have a significant subpopulation differentiation with an F ST value of 0.470. One 22 isolate was identified as a recombinant and the other 35 recombination-free isolates could be 23 divided into two major clades under different evolutionary constraints with ω-values of 0.055 24 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In 25 addition, the results from molecular variance of analysis (AMOVA) indicated that the effect of 26 host species on the genetic divergence of OrMV is greater than that of geography. In BaTS 27 analysis, OrMV isolates from the genera Ornithogalum, Lachenalia, Diuri tended to group 28 together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation. 29 Furthermore, age calculations suggested that the first divergence event of the OrMV isolates 30 analyzed might take place around 1068 BC.

Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety of ornamentals. In this study, the coat protein (CP) gene of OrMVwas used to investigate the molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two groups which have a significant subpopulation differentiation with an F ST value of 0.470. One isolate was identified as a recombinant and the other 35 recombination-free isolates could be divided into two major clades under different evolutionary constraints with ω-values of 0.055 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In addition, the results from molecular variance of analysis (AMOVA) indicated that the effect of host species on the genetic divergence of OrMV is greater than that of geography. In BaTS analysis, OrMV isolates from the genera Ornithogalum, Lachenalia, Diuri tended to group together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation. Furthermore, age calculations suggested that the first divergence event of the OrMV isolates analyzed might take place around 1068 BC. 17 Abstract 18 Ornithogalum mosaic virus (OrMV) has a wide host range and affects the production of a variety 19 of ornamentals. In this study, the coat protein (CP) gene of OrMV was used to investigate the 20 molecular mechanisms underlying the evolution of this virus. The 36 OrMV isolates fell into two 21 groups which have a significant subpopulation differentiation with an F ST value of 0.470. One 22 isolate was identified as a recombinant and the other 35 recombination-free isolates could be 23 divided into two major clades under different evolutionary constraints with ω-values of 0.055 24 and 0.028, respectively, indicating a role of purifying selection in the differentiation of OrMV. In 25 addition, the results from molecular variance of analysis (AMOVA) indicated that the effect of 26 host species on the genetic divergence of OrMV is greater than that of geography. In BaTS 27 analysis, OrMV isolates from the genera Ornithogalum, Lachenalia, Diuri tended to group 28 together, indicating that OrMV diversification was maintained, in part, by host-driven adaptation. 29 Furthermore, age calculations suggested that the first divergence event of the OrMV isolates 30 analyzed might take place around 1068 BC.