It is well established that dietary protein supply can influence the peri-parturient breakdown of immunity to nematode parasites but there is no information on the importance of exposure to nematode larvae during pregnancy for this response. We investigated this by exposing housed pregnant sheep, scanned as carrying two lambs, to a vaccinating infection with a trickle mixed infection of Teladorsagia circumcincta and Trichostrongylus colubriformis larvae (L3) or to no infection during weeks 29 to 24 relative to parturition. At the beginning of week 23 all sheep were treated with anthelmintic to remove any vaccinating worm burden and from week 22 to week þ6 received a trickle challenge infection with the same nematodes. Within each vaccinating treatment there were two nutritional treatments (no. ¼ 20 per subgroup) designed to provide 1.5 or 1.0 and 1.3 or 0.8 of metabolisable protein (MP) requirement during pregnancy and lactation, respectively. Five ewes were necropsied during weeks þ1 and þ3 to measure worm burdens and mucosal inflammatory cells and the remainder maintained until week þ6. Serum levels of total, IgA and IgE antibodies against L3 antigen of each nematode were measured.Scanning errors and lamb losses resulted in some ewes carrying and/or rearing only one lamb. Numbers of lambs reared was therefore introduced as a treatment effect. Vaccinating infection delayed the peri-parturient rise in faecal egg count (FEC) by an average of 2 weeks but its effect on FEC during the first 6 weeks of lactation was smaller and less persistent than that of dietary MP supply and single-v. twin-suckling.Populations of both nematodes were lower in association with high MP supply, vaccination and single suckling. These changes were associated with increases in numbers of mucosal mast cells (MMC) as a result of both increased MP supply and vaccination. Evidence for a more rapid return of host ability to limit populations of the abdominal nematode T. circumcincta than of the intestinal nematode T. colubriformis was associated with fewer eosinophils and more globule leucocytes (GL) in abomasal than in intestinal tissue.None of the serum antibody isotypes was affected by dietary protein supply. Total and IgA antibodies were maintained by a current larval (vaccinating) intake. IgA titres, however, increased progressively during pregnancy, especially in twin-bearing ewes. IgE titres appeared to be sensitive primarily to the reproductive cycle itself, peaking around parturition.This work supports the conclusion that availability of MP supply influences the recruitment and activity of cells of the immune armoury of the gastro-intestinal tract to nematode parasites. The precise outcome may differ with site and/or nematode species.

Sixty twin- and 60 single-bearing ewes were used in a 2 ✕ 2 ✕ 2 factorial design incorporating nutritional and shearing-cold exposure treatment (no. = 15 per subgroup) to study the effects on periparturient immunity against nematode infection. From week –8 to week –4 relative to parturition, animals grazed ryegrass-white clover pastures at allowances designed to provide either 0·8 (low allowance) or 1·2 (high allowance) of estimated metabolizable energy requirement for a ewe carrying 1·5 lambs. During week –4, half the sheep from each reproductive effort and nutritional group were shorn and exposed to artificial wind and rain for 4 h on each of 4 days consecutively. During the same week, all animals were challenged twice with 1·5 ✕ 104 Teladorsagia circumcincta and 1·5 ✕ 104 Trichostrongylus colubriformis infective third stage larvae to supplement the natural infection from pasture. From week –3 to week + 5 relative to parturition the sheep were run together and offered pasture at rates estimated to enable them to meet their nutrient requirement.Shearing and cold stress reduced ewe body weight (by up to 4·66 kg per sheep), and temporarily reduced the levels of IgA against T. circumcincta (by proportionately 0·24) and T. colubriformis (0·34), and raised faecal egg count (1·6 to 4·4 fold) but did not affect the parasite-specific total antibody levels in the serum. The low allowance during pregnancy was associated with a 6·09-kg decrease in ewe body weight, decrease in the levels of total antibody and of IgA against both T. circumcincta and T. colubriformis, and increased faecal egg count (by 1·5 to 10·6 fold) during pregnancy and lactation. Faecal egg count was consistently higher (up to 8·8 fold) in twin-bearing and rearing ewes than in single-bearing and rearing ewes except during the first 4 weeks of the experiment. A significant effect of litter size on antibody levels was relatively small when compared with short-term changes (reductions) in antibody levels immediately around parturition. There were significant negative correlations between faecal egg count during late pregnancy and ewe body weight in lactation and significant negative correlations between the levels of total antibody against T. colubriformis and faecal egg counts near the end of the experiment. We conclude that litter size is likely to have greater influence on the resistance of ewes to nematode infections in the periparturient period than either pasture allowance or cold stress within the range of parameters used in this work.

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A plasmid DNA containing the inserted Babesia bovis cDNA clone designated c51A was used to prepare a DNA probe for B. bovis. The purified 0.6 kb specific DNA fragment was labeled by DIG DNA labeling. After denaturation, the probe was hybridised with the blotted target DNA extracted from bovine red blood cells infected with B. bovis or other protozoa or bovine red blood cells plus extra white blood cells. It was found that the probe produced from purified 0.6 kb DNA fragment could detect sample of B. bovis DNA equivalent to 0.015 microliter of 10% whole infected blood. Compared to the same DNA fragment labeled with photobiotin, this new probe is more sensitive giving a darker hybridization signal, a lighter hybridization background and without any non-specific reactions. These results indicated that this DIG-labeled B. bovis C5lA probe could provide a sensitive and specific method to diagnose clinically suspected B. bovis infections and distinguish B. bovis DNA from other haemoprotozoan infections.