Purpose To investigate the effects of microRNAs‐107 (miR‐107) on autophagy, proliferation, and migration of breast cancer cells and its mechanism by targeting high mobility group protein B1 (HMGB1). Methods Real‐time polymerase chain reaction assay was used to detect the expression of miR‐107 in breast cancer and its cell lines. In MDA‐MB‐231 and MDA‐MB‐453 breast cancer cells, the expression of p62, Beclin1 protein, and the changes of cell proliferation and migration after overexpression of m miR‐107 were detected by Western blotting, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, and transwell assays. Target Scan online prediction, dual luciferase reporter gene, and Western blot were used to verify the targeting relationship between miR‐107 and HMGB1. The effects of silencing HMGB1 expression on p62, Beclin1 protein expression, cell proliferation, and migration ability were detected. The transfected MDA‐MB‐453 cells were inoculated into the right axilla of the nude mice, the tumor volume and weight were weighed, and the expression of miR‐107, HMGB1, p62, and Beclin1 in the tumor were detected. Results The expression of miR‐107 was downregulated in breast cancer tissues and cell lines (P < 0.01). The expression of p62 protein was upregulated (P < 0.01), while Beclin1 protein was downregulated (P < 0.01) and cell proliferation and migration ability were decreased (P < 0.01) after overexpressing miR‐107 in MDA‐MB‐231 and MDA‐MB‐453 cells. The results of TargetScan online prediction, dual luciferase reporter gene, and Western blot showed that miR‐107 could regulate HMGB1 expression. The expression of p62 protein was upregulated (P < 0.01), while Beclin1 protein was downregulated (P < 0.01) and cell proliferation and migration ability were decreased (P < 0.01) after silencing HMGB1 in MDA‐MB‐231 and MDA‐MB‐453 cells. The results of xenograft experiments showed that miR‐107 could delay tumor growth and inhibit autophagy. Conclusion miR‐107 could inhibit cell autophagy, proliferation, and migration of breast cancer cells by targeting HMGB1.

The aim of the study was to investigate the anti-tumoral activity of morelloflavone substances with different structures. We also studied the possible link between morelloflavone structure and its function. Various types of chromatographic techniques were used to isolate and screen morelloflavone substances from the extracts of gambogic tree trunk and the morelloflavone structures were identified by analytical techniques such as high resolution mass spectrometry and nuclear magnetism. Anti-tumoral activities of different compounds were investigated and the link between the antitumor activity and the structure of compound was exaimed. Our results showed that the isolated morelloflavone substances demonstrated a certain level of antitumor activity. The compound no. 1 had the strongest effect to inhibit glioma U87 and C6 cells followed by compound no. 2 while compound no. 5 was the weakest among them. We conducted a preliminary analysis on the structure-function relationship through the structure comparison and we concluded that the antitumor effects of morelloflavone substances with different structures were significantly different from each other. Thus, the glucose chain in C4 position of biflavone structure can enhance the antitumor activity of the compound in glioma cells. Additionally, the formation of intramolecular hydrogen bonds in biflavone compounds may also play a role in enhancing the antitumor activity and inhibition rate.

Objective: To explore the effect of the cluster nursing mode combined with blood pressure regulation on the surgical tolerance of patients with esophageal cancer and hypertension. Methods: The clinical data of 86 patients with esophageal cancer and hypertension treated in our hospital (February 2016-February 2017) were retrospectively analyzed. The patients were randomly split into research group and reference group, with 43 cases in each group. The reference group received routine nursing, while the research group received cluster nursing combined with blood pressure regulation. The SBP, DBP and heart rates at D1 (at admission), D2 (2 h before surgery) and D3 (2 h after surgery) were compared between the two groups. In addition, the surgical tolerance, ESCA scores at 1 month after discharge, personality and behavior characteristics before and after intervention, and incidence of postoperative complications were also compared between the two groups. Results: With no obvious difference in SBP, DBP and heart rates at D1 between the two groups (P > 0.05), the SBP, DBP and heart rates at D2 and D3 in the research group were obviously lower compared with the reference group (P < 0.001). With no obvious difference in the number of patients with surgical tolerance of Grades I, II and III after intervention between two groups (P > 0.05), the number of patients with surgical tolerance of Grade IV in the research group was significantly higher than that in the reference group (P < 0.05). The scores of self-concept, self-care skills, self-care responsibility and health knowledge in the research group at 1 month after discharge were obviously higher compared with the reference group (P < 0.001). The scores of EPQ-L (lie), EPQ-P (psychoticism) and EPQ-N (neurosism) after intervention in both groups were obviously lower compared with those before intervention (P < 0.001), and the scores of EPQ-L, EPQ-P and EPQ-N after intervention in the research group were significantly lower than those in the reference group (P < 0.001). The total incidence of postoperative complications in the research group was significantly lower compared with the reference group (P < 0.05). Conclusion: Cluster nursing combined with blood pressure regulation can significantly improve the surgical tolerance of patients with esophageal cancer and hypertension and enhance the self-care ability after surgery, with obvious therapeutic effect, which is worthy of promotion and application.

Breast cancer is a highly harmful malignant tumor, which poses a great threat to women’s body and mind, and the mortality rate ranks second among all women’s diseases. The incidence rate accounts for 7-10% of various malignant tumors in the whole body, second only to uterine cancer in women, and has become the main cause of threatening women’s health. Advanced breast cancer is often considered an incurable disease. The family of heterogeneous nuclear ribonucleoprotein complexes is composed of about 20 hnRNP proteins with molecular weights ranging from 32 to 120 kDa, and they are named according to their molecular weights. Among them, hnRNPA2 and hnRNPB1 are the two most important members of the hnRNP family, both derived from the same gene on chromosome 7p15. Therefore, research to understand the molecular mechanism and process of breast cancer progression has an important role in promoting the current medical research on breast cancer treatment methods. Therefore, studying the mechanism of tumorigenesis is the key to tumor prevention and treatment. Therefore, this paper proposes that A2/B1 promotes the stability of NRF2 mRNA and inhibits ferroptosis and cell proliferation in breast cancer cells. The article mainly introduces the disease diagnosis method based on artificial neural network and its neural network algorithm. In the experimental part, the activity of hnRNP A2/B1 on cancer cells is deeply studied. The results show that the absorbance of the MTT method increases continuously with the extension of the culture time, and the maximum reaches 1.2. This fully shows that its absorption capacity is very strong, especially after 24 hours, the absorption rate rises from 0.6 to 0.9, which shows that 24 hours is the best absorption time. And it can also be found that hnRNPA2/B1 has a significant inhibitory effect on breast cancer cells; it can reduce the effect on breast cancer cell cycle and apoptosis.