Citric acid cycle activity in mitochondria from mung bean (Phaseolus aureus var. Jumbo) hypocotyls were examined by surveying (a) Higher plant mitochondria exhibit many respiratory activities different from those of mammalian mitochondria (14, cf. 23). For example (a) plant mitochondria readily oxidize malate and externally applied NADH, whereas animal mitochondria oxidize malate poorly and do not oxidize externally applied NADH; (b) respiratory patterns in the oxidation of succinate and other substrates by plant mitochondria differ from those demonstrated with animal mitochondria; and (c) rotenone, by far the most potent inhibitor of NADH oxidase in animal mitochondria, is a weak and incomplete inhibitor of plant mitochondrial respiration. In order to characterize the respiratory properties of plant mitochondria further, current research is concerned with elucidating metabolic regulation of CAC6 activity in mung bean mitochondria.In contrast with several extensive studies on metabolic control of CAC activity in mammalian mitochondria (e.g. 27, 28) attempts to examine the over-all functioning of higher plant mitochondria (3, 13) have been sparse in recent years. In this com-

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56 human eyes were stored in a moist chamber from 0 to 4 days at +4°C. 20 human corneal buttons with a scleral rim were stored in the M-K medium for 5 and 7 days at +4°C. After this procedure in both series the temperature reversal effect was measured and the living endothelial cells were counted after being stained with trypan blue and rose bengal. All eyes showed a typical swelling-shrinking phenomena but after 4 days in a moist chamber this effect was definitely less characteristic than in all other periods of storage time. In respect to the endothelial cell counts 5 day M-K medium storage corresponds to 2 day moist chamber storage, and 7 day M-K storage is comparable to 3 day moist chamber storage. The total survival time of the endothelial cells in both series is almost equal.

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