No abstract

This study uses the Monte Carlo method and building performance simulations to develop an additive model for rapid peak load forecasting at design phase that considers the effects of design parameters. The Monte Carlo method generates numerous of simulation cases and EnergyPlus software is used for the calculations. Specifically, a total of 20 parameters were considered for analysing the peak load calculations, including design day conditions, envelope performance, infiltration, etc. An office building was selected as the reference building. With the screening experiments and the standard regression coefficient, it was identified that there are 15 important parameters for peak cooling load in the perimeter zones and 7 in the core zone. Main effects and interactions for selected parameters were determined by factorial experiments of 40,000 runs for the perimeter zone and 1,287 runs for the core zone. Main effects and interactions were used to develop an additive model between design parameters and peak cooling loads. Finally, model validation by additional 1,000 cases shows a coefficient of determination of 0.995, with a mean bias error of 3.2%, and a coefficient of variation of 3.7%, which indicated that the developed additive model had high accuracy.

A novel reusable biosensor of label-free biomolecular recognition based on the nano-mechanics transduction in a micro-fabricated cantiIever has been developed. The nanomechanics-based immunoassay biosensor was proven to be repeatedly used with desorption of the pre-bound antigen-antibody by an in-situ alternative electrical field. This new approach of electric manipulation in separating the antigen out of the antibody in a buffer fluid environment was confirmed in nanomechanics-based immunoassay biosensor in which a conventional desorption is long used with high concentrated acid solution in a range of pH 2-3. The potential benefit of the electrical means over a conventional pre-bound antigen-antibody desorption of strong acid solution is to maintain long-standing protein activity in multiple uses such that the micro biosensor is reliable and reproducible in every detection. The complete sensing history from a monolayer of bivlinker, to antibody and antigen is also fmt demonstrated in nanomechanics-based immunoassay.