No abstract

No abstract

Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.

Emergency department free-text chief complaints (CCs) are a major data source for syndromic surveillance. CCs need to be classified into syndromic categories for subsequent automatic analysis. However, the lack of a standard vocabulary and high-quality encodings of CCs hinder effective classification. This paper presents a new ontology-enhanced automatic CC classification approach. Exploiting semantic relations in a medical ontology, this approach is motivated to address the CC vocabulary variation problem in general and to meet the specific need for a classification approach capable of handling multiple sets of syndromic categories. We report an experimental study comparing our approach with two popular CC classification methods using a real-world dataset. This study indicates that our ontology-enhanced approach performs significantly better than the benchmark methods in terms of sensitivity, F measure, and F2 measure.

No abstract