Bex2
is well known for its role in the nervous system, and is associated with neurological disorders, but its role in the lung’s physiology is still not reported. To elucidate the functional role of
Bex2
in the lung, we generated a
Bex2
knock-out (KO) mouse model using the CRISPR-Cas9 technology and performed transcriptomic analysis. A total of 652 genes were identified as differentially expressed between
Bex2
-/-
and
Bex2
+/+
mice, out of which 500 were downregulated, while 152 were upregulated genes. Among these DEGs,
Ucp1, Myh6, Coxa7a1, Myl3, Ryr2, RNaset2b, Npy, Enob1, Krt5, Myl2, Hba-a2,
and
Nrob2
are the most prominent genes.
Myl2
, was the most downregulated gene, followed by
Npy, Hba-a2, Rnaset2b, nr0b2, Klra8,
and
Ucp1
.
Tcte3, Eno1b, Zfp990,
and
Pcdha9
were the most upregulated DEGs. According to gene enrichment analysis, PPAR pathway, cardiac muscle contraction, and cytokine-cytokine receptor interaction were the most enriched pathways. Besides, the nuclear factor-κB signaling pathway and hematopoietic cell linage pathways were also enriched. Chronic obstructive pulmonary disease (COPD) is enriched among KEGG disease pathways. RT-qPCR assays confirmed the RNA-Seq results. This study opens a new window toward the biological functions of
Bex2
in different systems.