Summary
Collagen fibres, stabilised by lysyl oxidase (LOX), play an important role in jumbo squid because they are responsible for the union between various cells; therefore, a close interdependence between their functions and muscle firmness during ice storage has been suggested. In this study, the relationship between LOX activity, pyridinoline (Pyr) content and muscle texture (SF) during ice storage of jumbo squid mantle was evaluated. LOX activity was confirmed within the range of 4.1–7.1 × 10−3 U g−1 of protein, leading to an increase in Pyr content, detected in the range of 0.85–1.32 mmol mol−1 of collagen after 5–20 days. The SF of the muscle became harder during the ice storage time, increasing from 21.08 to 37.95 N. It was therefore possible to establish the relationship between LOX activity, collagen cross‐links (Pyr content) and texture patterns during ice storage of jumbo squid muscle, which increased after 20 days.
Jumbo squid (Dosidicus gigas) is an important commercialized fishery in Mexico; however, during its post‐catch processing, several physicochemical changes occur in flesh, which could result in texture modifications. In this research, changes in water‐holding capacity, protein solubility (PS), firmness, fibrils microstructure, and color of squid muscle (mantle, fins, and arms) during cooking (30 min at 100 °C) were evaluated. Throughout cooking were exhibited different shrinkage and weight loss behavior among three anatomical regions (p < .05). After cooking, PS of muscle from fins and arms remained similar, meanwhile, mantle muscle PS decreased 75%. Shear force (SF) magnitude order (p < .05) registered in fresh muscle was fins > arms > mantle, whereas at ending cooking, mantle muscle required the lowest SF. Microscopic analysis revealed a major quantity of collagen fibers in fins and arm muscle suggesting its possible relation to their hardness, in both fresh and cooked state.
Practical applications
Jumbo squid (Dosidicus gigas) is an important marine resource in Mexico and many Asiatic and European countries. Its muscle (fins, arms, and mantle) edible items are commercialized and consumed in fresh, brined, and cooked presentations. Squid muscle protein structures (fibril and stromal) are organized different than fish as well as beef, pork and poultry; therefore its textural, physicochemical and sensorial (quality parameters) changes induced by cooking are distinctive. Squid meat acceptance by consumers is related mainly by texture than the taste or color attributes. Therefore, from economic point of view of domestic and large‐scale markets, the establishment of best cooking conditions of squid became essential.
In marine organisms primarily intended for human consumption, the quality of the muscle and the extracted oils may be affected by lipid oxidation during storage, even at low temperatures. This has led to a search for alternatives to maintain quality. In this sense, antioxidant compounds have been used to prevent such lipid deterioration. Among the most used compounds are tocopherols, which, due to their natural origin, have become an excellent alternative to prevent or retard lipid oxidation and maintain the quality of marine products. Tocopherols as antioxidants have been studied both exogenously and endogenously. Exogenous tocopherols are often used by incorporating them into plastic packaging films or adding them directly to fish oil. It has been observed that exogenous tocopherols incorporated in low concentrations maintain the quality of both muscle and the extracted oils during food storage. However, it has been reported that tocopherols applied at higher concentrations act as a prooxidant molecule, probably because their reactions with singlet oxygen may generate free radicals and cause the oxidation of polyunsaturated fatty acids in fish oils. However, when tocopherols are included in a fish diet (endogenous tocopherols), the antioxidant effect on the muscle lipids is more effective due to their incorporation into the membrane lipids, which can help extend the shelf life of seafood by reducing the lipid deterioration that occurs due to antioxidant synergy with other phenolic compounds used supplements in fish muscle. This review focuses on the most important studies in this field and highlights the potential of using tocopherols as antioxidants in marine oils.
The products of fishing are highly perishable foods. Thus, it is necessary to seek viable alternatives that help to preserve product freshness and quality and to increase its shelf life. One of these alternatives is the use of extracts with antimicrobial activity obtained from plants, such as tomato, which has been observed to present compounds with antimicrobial activity. The objective of this work was to determine the effect of an extract obtained from the tomato plant and incorporated into an edible chitosan coating on the quality and shelf life of the sierra fish fillet stored on ice for 15 days. For the latter, an extract was prepared with dehydrated tomato plants, which was incorporated by immersion alone or in combination with an edible chitosan-based coating in sierra fish fillets. The following treatments were applied: C (Chitosan), TPE (Tomato Plant Extract), TPE-C (TPE-Chitosan), and control (without chitosan and extract). Color, pH, WHC, ATP-related compounds, and K value were monitored during 15 days of storage on ice. Likewise, the total count of mesophiles was determined. The results indicated that treatments C, TPE, and TPE-C were significantly different (p<0.05) from lot control in terms of the concentration of ATP-related compounds, K value, pH, and total mesophilic count. We concluded that application of the extract alone or in combination with chitosan allows for the improvement of the quality and shelf life of the sierra fish fillet.
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