Berberine is a promising botanical pesticide against fungal plant pathogens. However, whether berberine inhibits the invasion of fungal pathogen across plant surface remains unclear. Here we demonstrated that the enzyme activities of purified cutinase from fungal pathogen Colletotrichum capsici were partially inhibited in presence of berberine toward different substrates. Molecular dynamics simulation results suggested the rigidity of cutinase was decreased with berberine added into the system. Interestingly, aggregations of berberine to the catalytic center of cutinase were observed, and stronger hydrophobic interactions were detected between key residue His 208 and berberine with concentrations of berberine increased. More importantly, this hydrophobic interaction conferred conformational change of the imidazole ring of His 208, which swung out of the catalytic center to an inactive mode. In summary, we provided the molecular mechanism of the effect of berberine on cutinase from C. capsici.

This study investigated the altering effect of moisture on the emission pattern of gases and the evolutionary dynamics of physicochemical indices in corn straw and cow manure composting. Exploring this effect was reasonable to unravel the use of moisture as a cheap alternative to control gaseous emissions and improve the final properties of compost. The nutrient dynamics of the compost showed 21.6% losses in total organic carbon content, with a 33.3% increase in total nitrogen content at the end of composting. All the gases (CH4, CO2, N2O and NH3) yielded a common emission pattern despite the differences in moisture content. Except for CH4, the peak and stable emission periods of all the gases were observed on the 5th day (thermophilic phase) and after the 27th day (late mesophilic phase) of composting, respectively. Emission reductions of 89%, 91%, 95% and 100% were recorded for CH4, CO2, N2O and NH3, respectively, during the late mesophilic phase of composting. From the study, the 65% moisture content was efficient in reducing the loss rate of the gasses and nutrient contents of the compost. This study would enable farmers to channel organic residues generated into compost while minimizing pollution and nutrient losses associated with the composting process.

Humic substances affect compost stability and maturation. However, the intricate structure of lignocellulosic materials hinders the biodegradation of cellulose, hemicellulose, and lignin, often promoting the use of synthetic additives which results in microbial inactivation and death. Therefore, this study examined the effects of optimal moisture levels (MC1 = 45%, MC2 = 55%, and MC3 = 65%) on lignocellulosic and humification fractions in aerobically composted straw and manure. The study showed that 65% moisture content was more efficient in decomposing cellulose, hemicellulose, and lignin, with hemicellulose (115.3% w/w ≈ 47.1%) degrading more than cellulose (76.0% w/w ≈ 39.5%) and lignin (39.9% w/w ≈ 25.9%). However, in compost heaps with 45% moisture, the humic acid concentration increased significantly by 12.4% (3.1% w/w) and 17.3% (4.3% w/w) compared with 55% and 65% moisture, respectively. All moisture levels increased the mineralization of humic substances, but the index measured was highest at 65% MC (23.8% w/w) and lowest at 45% MC (18% w/w). In addition, the humification rate showed the trend: 0.083% w/w > 0.087% w/w > 0.100% w/w for MC1, MC2, and MC3, respectively. Overall, the results indicate that an initial moisture content of 65% is aerobically efficient for the conversion of corn straw and cow manure into stable and mature compost.

Lignocellulosic materials have a complex physicochemical composition and structure that reduces their decomposition rate and hinders the formation of humic substances during composting. Therefore, a composting experiment was conducted to evaluate the effects of different C/N ratios on lignocellulose (cellulose, hemicellulose and lignin) degradation and the activities of corresponding enzymes during aerobic composting. The study had five C/N ratios, namely, T1 (C/N ratio of 15), T2 (C/N ratio of 20), T3 (C/N ratio of 25), T4 (C/N ratio of 30) and T5 (C/N ratio of 35). The results showed that treatments T3 and T4 had the highest rate of degradation of cellulose and hemicellulose, while treatment T3 had the highest rate of degradation of lignin. Among the five treatments, treatment T3 enhanced the degradation of the lignocellulose constituents, indicating a degradation rate of 6.86–35.17%, 15.63–44.08% and 31.69–165.60% for cellulose, hemicellulose and lignin, respectively. The degradation of cellulose and lignin occurred mainly at the thermophilic and late mesophilic phases of composting, while hemicellulose degradation occurred at the maturation phase. Treatment T3 was the best C/N ratio to stimulate the activities of manganese peroxidase, lignin peroxidase, polyphenol oxidase and peroxidase, which in turn promoted lignocellulose degradation.

Photocatalytic degradation technology has developed rapidly in the treatment of organic pollutants due to its high efficiency, mild reaction conditions and easy control. In this paper, a series of heterogeneous photocatalysts, BWZ-en-R (BWZ = [BW11Z(H2O)O39]7−, Z = Zn, Cd, Mn, en = ethylenediamine, R = Merrifield resin), were prepared by using ethanediamine as a linker to immobilize Keggin-type transition elements substituting tungstoborates on Merrifield resin and characterized by Fourier transform infrared spectroscopy, X-ray powder diffraction, scanning electron microscopy and energy-dispersive X-ray spectroscopy. The photocatalytic properties of BWZ-en-R (Z = Zn, Cd, Mn) for the degradation of methyl red (MR) were investigated. The results show that the BWZ-en-R (Z = Zn, Cd, Mn) photocatalysts exhibited high photodegradation ability for MR under the irradiation of ultraviolet light, and were easily separated from the reaction media. The maximum degradation rate (%) of MR (40 mL, 25 μM, pH = 2) reached 96.4% for the BWMn-en-R photocatalyst (40 mg) after being irradiated for 30 min, making this a promising photocatalyst candidate for dye degradation. Moreover, the influences of some factors, such as the Z-substituted elements in the BWZ, the BWZ-en-R dosage and the MR initial concentration, on the photocatalytic degradation rate of MR were also examined.