The functions of centrosomal protein ninein may be involved in microtubule minus end capping, centriole positioning, protein anchoring and microtubule nucleation, but the true physiological function of various human hNinein isoforms remains to be determined. Here we describe the identification of four diverse CCII-termini of human hNinein isoforms, including a novel isoform 6, by differential expression in a tissue-specific manner. These hNinein isoforms exhibit centrosomal (concentrated) and noncentrosomal (aggregated) localization when GFP-tagged fusion proteins are expressed transiently in mammalian cells. In a kinase assay, we show that the CCII region of hNinein provides a differential phosphorylation site by GSK3beta. In addition, our data indicate that either N-terminal or CCIIZ domain disruption may cause hNinein conformational change which recruits gamma-tubulin to centrosomal or noncentrosomal hNinein-containing sites, implying that the gamma-tubulin localization may be hNinein-dependent. Further, our RNA interference experiment against all hNinein isoforms caused a significant decrease in the gamma-tubulin signal in the centrosome. In domain swapping, we clearly show that the CCIIX-CCIIY region provides docking sites for gamma-tubulin. Moreover, our data also show that nucleation of microtubules from the centrosome is significantly affected by the presence of either the full -length hNinein or CCIIX-CCIIY region overexpression. Taken together, these results show that the centrosomal targeting signals of hNinein have a role not only in regulating hNinein conformation, resulting in localization change, but also provide docking sites to recruit gamma-tubulin at centrosomal and noncentrosomal sites.

The global innovation of information technology fundamentally reshaped the finance technology and the flourishing development over the past decade. The high coverage of both the internet network and the mobile phone has facilitated Taiwan to become one of the most potential internet finance regions. This study aims to empirically validate the technology acceptance model (TAM 3) for exploring the key factors that influence the internet finance development. SEM was adopted to investigate both the hypothesis and the path coefficient associations among the constructs and their significance. Confirmed factor analysis for the instrument validity and reliability assessment was made. Hierarchy regression was performed to test the moderator effect of experience and voluntariness. This study result found that Taiwan finance customers had high expectations of information security and preferred high technology products with complex functions. The result demonstrated that customer's Use Behavior was influenced by Perceived of Usefulness, not Perceived of Ease of Use. The moderator effect result in Experience had a positive moderator effect on Objective Usability to Perceived Ease of Use. This research extracted the key factors that influenced the internet finance development in Taiwan for providing both local government and internet finance developing countries guidelines while concurrently developing an internet finance development strategy.

Purpose The purpose of this paper is threefold: developing an effectiveness communication model for a city rotary event. Empirical validation of the new effectiveness model based on a city cultural festival event to explore the similarity and difference of perception change on event attendance. Providing a structural equation modeling (SEM) analysis of communication model for both professional event organizers and PCO guidelines when determining an event’s marketing strategy priorities. Design/methodology/approach The authors use SEM to verify the relationship between variables, and path coefficient comparison analysis which was adapted for model verification and hypothesis testing. Analytical methods included reliability analysis, descriptive statistical analysis, Hotelling T 2-test, SEM analysis. Findings This result demonstrates that attendee increased their emotion, attitude, city brand image as result of the event activities, and positive event emotions have the highest influence toward to a positive city’s brand emotion. A positive city brand emotion especially has the second highest influence on attendee next event retentions. A positive city brand emotion will lead to a positive city brand attitude, but city brand attitudes will not have a positive impact on retentions. Originality/value The contribution and value of this study is that the authors established a new effectiveness communication model which introduce the city branding concept into the research. The study provides empirical evidence of the effectiveness illustrations of the application in the communication model. The research meets the objective in this effectiveness communication model. It illustrates the directions for how an event should be designed in order to create a positive city brand attitude for retention.

This paper, evaluates and compares the heterogeneous balancevariation order pair of any two decision-making trial and evaluation laboratory (DEMATEL) theories, in which one has a larger balance and a smaller variation. In contrast, the other one has a smaller balance and a larger variation. With this said, the first author proposed a useful integrated validity index to evaluate any DEMATEL theory presence by combining Liu's balanced coefficient and Liu's variation coefficient. Applying this new validity index, three DEMATELs kinds with a same direct relational matrix are compared that are: the traditional, shrinkage, and balance. Furthermore, conducted is a simple validity experiment Results. show that the balance DEMATEL has the best performance. And that, the shrinkage coefficient's performance is better than that of the traditional DEMATEL.