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This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.

A comparative study on the effect of oxytocin, extracted leaf of katu (Sauropus androgynous, Merr) and lampes (Ocimum sanctum, Linn) against secretions of milk and histologically mammary gland of mice was carried out under laboratory conditions. The study was designed by using totally 40 mice pp and divided into four groups treatment which consisted 10 females mice post partum (pp) of each group. The control group, katu (S.androginus), lampes (O. sanctum), and oxytosin were given orally 0.5 ml physiological solution, 10 mg/0.5 ml leaf extract of S. androgynus , 10 mg/0.5 ml leaf extract of O. sanctum and 0.1 IU oxytocin intramuscular, respectively. The treatment were started on day fourt to 21th of lactation period. The data were observed on day 6th, 9th, 12th, 15th, of lactation period and than on day 21th the mice were killed to prepare histologically of mammary glands. The data analyzed by ANOVA and LSD test. The result of the study showed that the secretion of the milk on the mice were not significantly different among of fourth groups. However between S. androgynous and O. sanctum group accured an increasing on secretion of milk productions. The diameter of alveolus pit of mammae lobus gland were not significantly different amongs the fourth group, but the number of alveolus of mammae lobus gland were significantly different among oxytocin, S. androgynous, and O. sanctum group and between the group of O. sanctum and control.

Research has done to investigate the effect of pumpkin seed extract on morphology and motility of the mices after given of 2-ME. Thirty male mices, 56-63 days with 23-27 g weight were used as experimental animals into 5 groups (2 groups is control and 3 groups is treatment), 6 mices each. The fi rst group is K 0 which given 0.1 mL aquades by gavage for 40 days each day, and then K 1 were given 0.05 mL 2-ME 200 mg/kg by intraperitoneally everyday within 5 days, and continued on the giving of aquadest. The next groups are treated groups which given 2-ME, in the fi rst 5 days, then continued to the givings of pumpkin seed extract by gavage. Cucurbita moschata seed extract were given in three doses: 0.5; 1.0; and 2.0 g/kg BB for 35 days. Sperm collected from cauda epididymis. Parameter of sperm quality are morphology and motility. The result shown that Cucurbita moschata seed extract doses 0.5-1.0 g/kg BB showed no signifi cant effect and then doses 2.0 g/kg BB increased recovery process morphology and motility with signifi cant differences p < 0.05. The conclutions were Cucurbita moschata seed extract increased recovery process morphology and motility of the mices.

This study was aimed to know the effects of oyster mushroom supplement to milk secretion and mammary alveolus diameter of mice. The research was carried out under laboratory condition at the laboratory of Reproduction Biology, Faculty of Mathematic and Sciences, Airlangga University by using Complete Random Method. The study was designed by using totally 32 mice post partum (PP) and devided into four groups of treatment which consisted 8 female mice post partum of each group. The groups are: (a) the control groups, the groups that were given 0.2 ml aquadest, (b) the treatments of oyster mushroom solution with 3 variation of mushroom concentration, there are 2, 4 and 6 percent. The oyster mushroom solution was given by gavage. The treatments were started on 3th to 12th day of lactation period. The increasing of milk secretion were showed by the increasing of body weight of offsprings. The data were collected from the difference result of the balance of weight body of offspring and the data were observed on 4th, 6th, 8th, 10th, and 12th of lactation periode and then on 13th day, the mice (five mice for each group) were killed to make histological preparat of mammary glands. The datas were analyzed by ANOVA and LSD test (a = 0.05). The results of this research showed that oyster mushroom supplement can increase milk secretion and mammary alveolus diameter of mice. Oyster mushroom with 6 percent concentration gave the best effects and there was a positive corelation between the weight of oyster mushroom that was used with the increasing of the oyster mushroom effects.