EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus)

Hayati, Alfiah; Yunaida, Binti; Pidada, I.B. Rai; Darmanto, Win; Winarni, Dwi

doi:10.23869/bphjbr.10.1.20042

Cited by 4 publications

(6 citation statements)

References 7 publications

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“…This condition can also be caused by 2-ME. This statement is supported by the results of previous research which states that exposure to 2-ME 200 mg/kg BW resulted in the destruction of seminiferous tubules, decreased spermatogonium and spermatid levels and damaged spermatozoa morphology (Hayati et al 2004).…”

Section: Discussionsupporting

confidence: 80%

“…The 2-ME compound is not found naturally in environment because its presence in nature is the result of industrial and factory activity. The 2-ME compounds may cause decreased motility and morphology of spermatozoa (Hayati et al 2004). The 2-methoxyethanol (2-ME) compound is one of the toxic materials that potentially damage male reproductive organs.…”

Section: Introductionmentioning

confidence: 99%

“…The accumulated toxic substances can disrupt human health with various metabolic barriers. 2-ME exposure of 200 mg/kg BW causes damage to seminiferous tubules, decreases spermatogonium and spermatid levels and can damage spermatozoa (Hayati et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME)

Sitaresmi¹,

I’tishom²,

Mustika³

2017

FMI

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The compound of 2-methoxyethanol (2-ME) is one of toxic materials that potentially damage male reproductive organs. This compound can enter the body by various means of direct contact through the skin, respiratory and digestive tract. This compound can cause oxidative stress in spermatozoa which is the main cause of spermatozoa dysfunction. Lycopene, also known as red pigment, is an antioxidant class of carotene. Lycopene has antioxidant activity twice stronger than beta carotene and ten times stronger than vitamin E. Thus, the lycopene reaction as an antioxidant in the body is better than vitamin A, C, E, and other minerals. This study was conducted to determine the effects of lycopene on spermatozoa morphology in mice exposed to 2-ME. The study was conducted on 30 mice, divided into 5 groups. The K-group was the control group without 2-methoxyethanol and lycopene, the K + group was the group with 200 mg/kg BW 2-methoxyethanol on days 1-5, and the P1, P2 and P3 groups were the ones with 200 mg/kg BW 2-methoxyethanol on days 1-5 and lycopene doses of 5, 10 and 20 mg/kg BW on days 6-35. 2-ME was given intraperito-neally and lycopene was given per sonde. On day 36, the mice were sacrificed and the epididymis and vas deferens were removed for morphological examination of spermatozoa. The observations were performed using a microscope with 1000x magnification. The results showed that there was significant difference. In the morphology of spermatozoa, the administration of lycopene increases normal morphological percentage of spermatozoa in mice exposed to 2-ME.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME)

Sitaresmi¹,

I’tishom²,

Mustika³

2017

FMI

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“…Sel spermatosit dan spermatid merupakan sel spermatogenik yang rentan terhadap perubahan fisiologis testis akibat paparan lingkungan karena spermatosit khususnya spermatosit pakiten sedang aktif mensintesis RNA. Berbeda dengan sel spermatosit dan spermatid, spermatogonia lebih tahan terhadap paparan karena sel ini terletak pada membran basal yang terlindung oleh barrier yang dibentuk oleh sel sertoli (9). Sel spermatosit dan spermatid yang terdapat didalam tubulus seminiferus dapat menjadi pedoman ada tidaknya penghambatan proses spermatogenesis.…”

Section: Hasil Dan Pembahasan Diameter Tubulus Seminiferus (Dts) Dan unclassified

Pengaruh Ekstrak Ethanol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan

Anzila

Pramana²,

Soewondo³

et al. 2017

Biotropika

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ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh ekstrak ethanol kemangi (Ocimum canum Sims.) terhadap struktur histologi testis mencit jantan (Mus musculus). Penelitian ini menggunakan 9 ekor hewan coba mencit strain Balb/c jantan berumur 3 bulan, berat badan 20 -30 g. Hewan coba dibagi menjadi tiga (3) kelompok kontrol tanpa pemberian ekstrak kemangi (P0), kelompok yang diberi ekstrak kemangi dengan dosis 100 mg/kgBB (P1) dan 200 mg/kgBB (P2). Ekstrak kemangi diberikan setiap hari secara oral selama 35 hari. Parameter yang diamati adalah diameter tubulus seminiferus, tebal epitel germinal, jumlah lapisan germinal dan jumlah sel spermatogenik meliputi spermatogonia, spermatosit dan spermatid. Data yang diperoleh dianalisis dengan One-way ANOVA (p ≤ 0,05). Jika menunjukkan perbedaan yang bermakna, maka dilanjutkan dengan uji Least Significant Difference (LSD). Hasil penelitian menunjukkan bahwa pemberian ekstrak kemangi dosis 200 mg/kgBB meningkatkan diameter tubulus seminiferus, tebal epitel germinal, dan jumlah sel spermatid.Kata kunci : Ocimum canum, spermatid, tubulus seminiferus ABSTRACTThe research was conducted to observe the effect of kemangi ethanol extract (Ocimum canum Sims.) to the testis histology structure of male mice (Mus musculus). Research materials were using 9 BALB/c male mice on 3-4 months age with 20-30 g, which divided into 3 group (each group is 3 mice) is K (control), P1 (with 100 mg/kgBW of kemangi extract dose), P2 (with 200 mg/kgBW of kemangi extract dose). The extract was given orally every day for 35 days. On the 36 th day, the animal model was sectioned for a testes organ isolation. Testes washed with PBS and fixed with 10% formalin for 24 hours, then made the histology of testes by paraffin method and Hematoxylin-Eosin staining. Observed parameters were the tubules seminiferous diameter, the thickness of germ epitel, the amount of germ layer and spermatogenic cell (spermatogonials, spermatocytes, and spermatids). The data were analyzed with Oneway ANOVA (p ≤ 0,05). If showed a significant difference, then continued with Least Significant Difference (LSD) Analysis. The results showed that giving 200 mg/kgBW of kemangi extract dose increased the tubulus seminiferous diameter, the thickness of germ epitel, and a number of spermatid cells. Key words : Ocimum canum, spermatid, seminiferous tubules PENDAHULUANInfertilitas merupakan gangguan reproduksi yang dapat dialami pria dan wanita. Infertilitas pada pria dapat disebabkan adanya gangguan pada proses spermatogenesis. Gangguan spermatogenesis dapat disebabkan oleh faktor internal (umur, psikologi, hormon dan genetik) atau factor eksternal. Sedangkan faktor eksternal (suhu, nutrisi, trauma, polusi lingkungan dan radiasi sinar X ) (1).Salah satu penyebab utama terganggunya proses spermatogenesis di dalam testis adalah adanya radikal bebas. Sumber radikal bebas ada dua yaitu endogen dan eksogen. Sumber radikal endogen adalah reaksi-reaksi yang ada di dalam tubuh misalnya autoksidasi, oksidasi enzimatik maupun respiratory burst. ...

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“…Oxidation stress is caused by the formation of reactive oxygen species (ROS). Very high levels of ROS not only affect the integrity of DNA in the nucleus of spermatozoa, but also the flexibility of cell membranes thereby reducing the quality of spermatozoa (Hayati et al 2004). Dates contain high antioxidants in the form of flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ethanol Extract of Date Palm Fruit (Phoenix dactylifera. L) on Spermatozoa Concentration of BALB/c Mice (Mus Musculus) Exposed to 2-Methoxyethanol

Yassin

Yaudiwati

I’tishom

2020

FMI

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Antioxidants are important compounds for the human body because they function to capture free radicals causing degenerative diseases. Flavonoid and phenolic compounds in dates have antioxidant activity that can inhibit the increase in lipid peroxide and protein oxide. This study aims to prove the increase in the concentration of mice (Mus musculus) spermatozoa given ethanol extract dates and exposed to 2-methoxyethanol. Experimental animals used 35 mice (Mus musculus), divided into 5 groups (7 mice per group). The negative control group (K-) was the control group without administration of 2-methoxyethanol and date ethanol extract, the positive control group (K+) was given 200 mg/kg 2-methoxyethanol + CMC 0.5%, the treatment group 1 (P1) was given 200 mg/kg BW 2-methoxyethanol + 3.5 mg/gBW of ethanol extract dates, treatment group 2 (P2) were given 200 mg/kg BW 2-methoxyethanol + 7 mg/gBW of date palm ethanol extract, and treatment group 3 (P3) were given 200 mg/kg 2-methoxyethanol + 10.5 mg/gBW of ethanol extract dates. The results showed there were significant differences in spermatozoa concentrations between the positive control group (K+) and the negative control group (K-), treatment group 1 (P1) and treatment groups 2 and 3. mice (Mus musculus) exposed to 2-methoxyethanol.

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EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus)

Cited by 4 publications

References 7 publications

EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME)

EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME)

Pengaruh Ekstrak Ethanol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan

Effect of Ethanol Extract of Date Palm Fruit (Phoenix dactylifera. L) on Spermatozoa Concentration of BALB/c Mice (Mus Musculus) Exposed to 2-Methoxyethanol

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