The effects of copper in solution on Icfalurus nebulosus was studied by means of acute short-term bioassay and long-term bioassay. Histomorphological and histochemical tests were performed on skin. liver, gills, gut and kidney; enzymatic tests were also performed on the liver.Morphological changes of skin. liver and gills are described. The results of the histoenzymatic tests on the liver agree with the histological findings and confirm a mild onset and slow evolution of liver distress.

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-Computerised image analysis on Feulgen-stained preparations indicated that in both Lophius piscatorius and Diodon holacanthus the Feulgen-DNA content of the hypothalamic magnocellular neurons in the preoptic and tuberal complexes largely exceeded 2C. Subsequent quantitative microfluorometric evaluation revealed that the DNA content of these cells was as higher as 68C in L. piscatorius and 84C in D. holacanthus. The presence of high levels of DNA content in neuron nuclei is well known in the case of the giant neurons of Molluscs but, although it has recently been found in the supramedullary neuron cluster of the same Teleosts used in this study, it must be considered exceptional for the nervous system of Vertebrates.

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