Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an E. coli K1 strain in a 10 L bioreactor in batch-and fed-batch mode, respectively. A new downstream process for polySia is presented, based on membrane adsorber technology and use of inorganic anion exchanger. These methods enable the replacement of precipitation steps, such as acetone-, cetavlon-and ethanol-precipitation of the already established purification process. The purification process was simplified, while process efficiency and product qualities were improved. The overall yield of polySia from a 10 L batch cultivation process was 61 % and for 10 L fed-batch cultivation process 40 % with an overall purity of 98 %. The endotoxin content was determined to be negligible (14 EU mg -1 ). Th e ma i n advantage of this new downstream process is that polySia with high chain length > 130 DP (degree of polymerization) can be obtained. In fed-batch cultivation chain lengths up to 160 DP were obtained.
Das anaerobe gastrointestinale Bakterium Eubacterium ramulus wurde erstmals in einem Einweg‐Beutelreaktorsystem mit 10 L Kulturmedium kultiviert. Im Vergleich zu Standard‐Flaschenkultivierungen mit Hungate‐Technik wurde um den Faktor 25 aufskaliert und die optische Dichte konnte um mehr als das 5fache gesteigert werden. Bei einer 10‐L‐Batch‐Kultivierung im Einweg‐Beutelreaktorsystem wurden 60 bis 70 g Feuchtzellmasse produziert. Zum Vergleich wurde eine 10‐L‐Batch‐Kultivierung in einem Edelstahlrührkessel durchgeführt, die ähnliche Ergebnisse wie das Beutelreaktorsystem hinsichtlich der Feuchtzellmasse und der optischen Dichte zeigte.
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