No abstract

No abstract

A new, sensitive and simple spectrophotometric method for the simultaneous determination of Sunset Yellow FCF and Sudan I in mixtures is proposed. The method is based on the isolation of Sunset Yellow FCF on Sephadex diethylaminoethyl (DEAE)A-25 gel (pH 5.0) and the isolation of the Sudan I on C18 silica gel @€I 5.0) measuring, directly, their absorbanas in the solid phase at 3, = 487 nm in both cases. The concentration ranges applicable were between 15 and 500 ng ml-1 for Sunset Yellow FCF and between 20 and 200 ng ml-1 for Sudan I with detection limits of 3.5 and 4.4 ng ml-1, respectively. The method was applied to the determination of mixtures of both compounds in synthetic colourings used as additives in food, drugs and cosmetic products.

A method for the simultaneous determination of the colorants Sunset Yellow FCF and Quinoline Yellow using solid-phase spectrophotometry is proposed. The colorants were isolated in Sephadex DEAE A-25 gel at pH 5.0, the gel-colorants system was packed in a 1 mm silica cell and spectra were recorded between 400 and 600 nm against a blank. Statistical results were obtained by partial least squares (PLS) multivariate calibration. The optimized matrix by using the PLS-2 method enables the determination of the colorants in artificial mixtures and commercial soft drinks.

No abstract