The study aimed to evaluat Bacillus subtilis biodegrdation one of organic-pesticides in different periods. Bacillus subtilis was isolated from Iraqi soils, identification by morphological and biochemical tests. The best growth of B. subtilis were in(72hours) / (10-15) ppm (0.200-0.196)respectively, while the 5 ppm showed the highest growth in 60 day (0.163). The best degradation rate% were for 15 ppm/14 days (90.32)%. The evluation of (N-phosphonomethyl)glycine residues concentration’s via HPLC and degradation ratio%, showed with increasing time incubation to 30 days, Bacillus subtilis degradation ratio% increased for (15)ppm, while the best 60 days / (25)ppm. From all the conclusion is that the B. subtilis used the Glyphosate as source for carbon and phosphorus and suggest could be well exploited for bioremediation of Glyphosate contaminated sites in 15ppm/30 days and 20 ppm/60 days.