Bioremediation of pesticides is the best option available to date due to its eco-friendly, cost-effective and efficacious nature. The study aimed to evaluate the Azotobacter spp. bioremediation Chemosate in the different incubation period and concentrations (5, 10, 15, 20, 25 ppm). From local sites, different microbes were isolated and Azotobacter separated using selective methods for identification of characteristics. The best result for the growth of Azotobacter sp. was at 25 ppm/0.222-0.163, in 15 days; in addition, the great degradation rate % was 25 ppm / 54.16%, observed in 2 months, while the best degradation and residues of chemosate after its digestion through MSM and HPLC residues analyses were at 25 ppm, as seen in 1-2 months, respectively. The degradation ratio % reached 81-79 % for 1-2 months. This conclusion suggests that Azotobacter spp. degradation Chemosate principles applied via hydrolysis binds phosphorus bonds with oxygen and digests the pesticides to produce nitrogen and carbon as elements for its growth sequences, especially at 2 months/25ppm.
Abstract-Bioremediation of polychlorinated biphenyls (PCBs) by anaerobic microbial dechlorination occurring naturally in the subsurface and in engineered systems results in mixtures of lower-chlorinated, primarily ortho-substituted biphenyls. The purpose of this study was to determine whether this process of bacterial dechlorination results in a mixture that differs in biological activity from that of the parent PCB mixture. Two biological assays sensitive to the action of ortho-substituted PCBs were employed: insulin release by RINm5F cells, and superoxide anion ( ) production by rat neutrophils. The PCB mixtures Aroclor 1242 and Ϫ
The study aimed to evaluat Bacillus subtilis biodegrdation one of organic-pesticides in different periods. Bacillus subtilis was isolated from Iraqi soils, identification by morphological and biochemical tests. The best growth of B. subtilis were in(72hours) / (10-15) ppm (0.200-0.196)respectively, while the 5 ppm showed the highest growth in 60 day (0.163). The best degradation rate% were for 15 ppm/14 days (90.32)%. The evluation of (N-phosphonomethyl)glycine residues concentration’s via HPLC and degradation ratio%, showed with increasing time incubation to 30 days, Bacillus subtilis degradation ratio% increased for (15)ppm, while the best 60 days / (25)ppm. From all the conclusion is that the B. subtilis used the Glyphosate as source for carbon and phosphorus and suggest could be well exploited for bioremediation of Glyphosate contaminated sites in 15ppm/30 days and 20 ppm/60 days.
This study was aimed to evaluate the Bacillus megatrium ability to growth and degradated the organophosphorus pesticides, Glyphosate. , Bacillus megaterium was isolated from Iraqi Soils and identification by morphological and biochemical tests beside a Sperber’s Medium as selectivity media. The best growth results were in (2- 60) days, had the same growth for both (5, 25) ppm on MSM. The best degradation rate ability % were in (25) ppm /60 days (70.9)%. The increasing in incubation show increasing of degradation ration% of Glyphosate via HPLC specially after 60 days , the best ration were for (25)ppm .The result is the B. megaterium used the Glyphosate as source for carbon and phosphorus and suggest could be well exploited for bioremediation of Glyphosate contaminated sites.
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