No abstract

Cloning and characterization of three cDNAs and one gene coding for the aspartic proteinase inhibitor homologues, detection of four protein isoforms and analysis of the gene copy number indicate that in potato aspartic proteinase inhibitors are encoded by a multigene family.

EMBL accession no. X53470 cDNA sequences of any aspartic proteinase inhibitors have not been reported so far. Two protein inhibitors of aspartic proteinases were isolated from potato tubers and sequenced (1-3). In order to find related inhibitors, several cDNA clones have been isolated from a potato tuber lambda gtl 1 cDNA library encoding aspartic proteinase inhibitor homologues. The longest, full-length clone contains an open reading frame of 660 bp coding for a protein of 220 amino acid residues. The deduced protein sequence shows about 94% and 99% similarity to the novel inhibitor of cathepsin D (3) and potato cathepsin D inhibitor (2), respectively. The presumed signal peptide and two polyadenylation signals are underlined.

No abstract

No abstract