The aim of the study was the preliminary development of laparoscopic transfer of embryos to the uterus in the pig, which can become the alternative for more invasive surgical methods. We proposed the original method of embryo transfer. Donors (n = 40) and recipients (n = 15) of embryos were sows of age of 6-8 months. The estrus cycle of both recipients and donors was routinely synchronized. The experimental animals were divided into two groups. In the first group (10 donors and 3 recipients) embryos were transplanted according to the method described earlier and in the second group (30 donors and 12 recipients) embryos were transplanted according to our own proposed method. As the control group, we used 16 sows after insemination (AI). In animals from both experimental groups pregnancy was diagnosed between 28-31 day after transplantation and in the control group between 28-31 day after insemination. All animals were observed during pregnancy and weaning period in pig farm. Embryos at the development stage of 2-4 cell were obtained surgically and cultured in vitro for 4 days. Obtained blastocysts were transferred to donors. The original set of catheters for blastocysts transfer to pig uterus was constructed. Three trocars were placed in abdominal cavity for inserting endoscope and 2 grasps for uterus stabilization. After uterus stabilization, the slide was inserted into abdomen which was used for putting the needle to puncture uterus. Through this needle catheter with embryos was inserted into the uterus cavity. Embryos were placed by injection into lumen of the one uterine horn. From 12 recipients pregnancy was diagnosed in 6 recipients. From 6 litters, 57 piglets were born. We weaned 41 piglets (71.9%). In our study we obtained 50% efficacy, with the mean number of 9.5 alive piglets in litter and 6.8 weaned piglets. The efficacy of developed method of laparoscopic transfer of porcine embryos allows it to be used in routine practice.

prolactin (prl) is a protein hormone synthesized in and secreted predominantly by lactotroph cells of the anterior pituitary gland. this hormone has been found to stimulate the immune system in animals. Because prolactin secreted in milk by the mother's body is often insufficient, the administration of exogenous prolactin may significantly contribute to improving the health and growth of piglets. The aim of the study was to determine the dose of prolactin administered to newborn piglets to improve their survivability and growth rate. The study used Biolactin solution, produced on a semi-technical scale by FZNP Biochefa, which was administered per os to newborn piglets at a dose of 0.1, 0.5 and 1.0 mg PRL/kg body weight. NaCl physiological saline was used as a control. The number of piglets born alive, piglet body weight and mortality from birth to 21 and 28 days of age (weaning) were monitored. The study involved 98 litters (1197 piglets), which were divided into 3 experimental and 3 control groups. The experiment showed a positive effect of exogenous prolactin administered to newborn piglets on reducing their mortality and on increasing their birth to weaning growth rate. A dose of 0.5 mg PRL/kg body weight turned out to be optimal.