Müllerová D., Landis M., Schiess I., Jablonický J., Prístavka M., 2011. Operating parameters and emission evaluation of tractors running on diesel oil and biofuel. Res. Agr. Eng., 57 (Special Issue): S35-S42.This work is aimed at the evaluation of the operating parameters and emission of two tractors: Hürlimann H-488 DT and Hürlimann XB Max 100. The measurements were done on a test bench in the laboratory of the Agroscope Reckenholz-Tänikon Research Station ART, Ettenhausen, Switzerland during February 2010. The goal of this paper was to evaluate the operating parameters of the two models of tractors by using classical diesel oil and biofuel, as well as to evaluate the emission (greenhouse gases, dangerous exhaust gases and carcinogens), to make statistical analysis of the results and the conclusion about the samples used and the impact on engine parameters, environment and human health. From the results achieved, it is possible to state the following facts. In each way, the emissions of rape seed methylester (RME) and diesel are equivalent. The values of CO and HC and also particles are lower for RME. But NO x values are lower for diesel oil. It is liquid that the newer engine of Hürlimann XB Max 100 decreases emission of CO, HC and NO x significantly.