The article presents the results of research and treatment of cats with chlamydia in Poltava. The most common clinical sign of feline chlamydia is conjunctivitis (57 %) and keratitis (9.5 %). The combination of keratitis with conjunctivitis was found in 6 animals (28 %). Laboratory diagnosis of chlamydial infection was based on detecting chlamydial structures in a light microscope. In mild cases, when studying smears after Romanowski staining, Galbedstedter-Provachek bodies, which are cytoplasmic inclusions characteristic of chlamydia, were found in the material. Inclusions were painted according to Romanovsky-Gimza in blue and contained large reticular bodies, which, and small elementary bodies, were painted in purple-red. They were usually located near the nucleus. In the early stages of development, the parasites reached only 2–4 μm in diameter. At some stages of inclusion, they had a larger diameter and occupied the entire cytoplasm of the cell. The cell nucleus was displaced to the periphery of the membrane and changed shape. In addition to typical inclusions, parasites were often detected at the stage of reticular bodies. As a rule, the cells showed signs of damage in such cases. The treatment of cats with chlamydia was comprehensive. Antichlamydial therapy combines antibacterial therapy and immunocorrection. Cycloferon 12.5 % (aqueous solution for injection) was used as an immunomodulator for treating chlamydia. It was administered intramuscularly at a rate of 10 mg/kg body weight once a day–a total of ten injections. The second course was carried out in three weeks. Application Cycloferon 12.5 % was combined with an antibacterial agent. Azithromycin 10% was used as an antibacterial agent. It was included in the treatment regimen from the second day of application of Cycloferon 12.5 % intramuscularly, 1 ml per 10 kg of body weight; for 14 days (every 72 hours). Vigamox eye drops were instilled one drop into each eye four times a day for 21 days. In persistent conjunctival hyperemia, 1 % Emoxipin was prescribed as a capillary stabilizing agent. It was administered subconjunctivally in two drops (0.2–0.3 ml) twice a day for 15 days. Prodevit was used as a vitamin preparation. It was administered subcutaneously in a dosage of 0.3 ml: once every seven days. In most animals, improvement in clinical condition was noted on days 13–15 after treatment. During this period, there were no signs of purulent conjunctivitis, breathing improved, and wheezing was not heard. In animals diagnosed at the initial stage of the disease, clinical recovery occurred 7–8 days after treatment.

For suspicion of an infectious disease of cats, diagnostic tests were conducted. From the purulent leakages of the nasal cavity of the cat, pure cultures of the Bordetal, Escherichia and Staphylococcus cultures were obtained. Differential environments were used for this purpose. For effective treatment, each of the isolated cultures was tested for antibacterial sensitivity. According to the results, isolated cultures showed the highest sensitivity to gentamicin and enrofloxacin. Given the sensitivity of the isolated microflora, enrofloxacin-50 was used in the range of 0.1 ml per 1 kg of body weight for 5 days subcutaneously. Cats are a kind of animals that are sensitive to antibacterial substances. The choice of enrofloxacin was due to the lack of reactions and sensitivity to this drug in cats. As the immunomodulatory agents, which increase the overall resistance of the body used Katozal and Gamavat. Katozal was used for cats once a day subcutaneously for 5 days, at a dose of 0.5 ml for 5 days. Gamavit was administered subcutaneously at a dose of 0.3–0.5 ml/kg body weight (therapeutic dose). Tetravit was used as a vitamin preparation. It was injected subcutaneously 0.3 ml; once in 7 days. Improvement of the clinical condition of the diseased animal was observed the second day after the start of treatment; the animal revived, appetite appeared, the mucus-purulent discharge from the nasal cavity stopped. As bordeliosis is transmitted by airborne droplet, air disinfection was carried out using a bactericidal irradiator BactoSfera OBB 36 P with a quartz lamp. To disinfect the bactericidal lamp was switched on every day – in the morning and evening for 20 minutes.

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The article presents epizootological data and clinical signs of feline chlamydia in Poltava. According to the results of studies in 2018–2020, a wide spread of chlamydia infection among cats in the city of Poltava was established. The largest number of the studied cats, were pedigree or crossbred. There was no explicit clear seasonality, but a greater number of cases of respiratory diseases were recorded in the autumn-winter period of the year. In the structure of infectious diseases during the research period, chlamydia mono-infection was registered in 68 % of cats, association of chlamydia with infectious rhinotracheitis in 18.5 %, kalitsivirus infection in 8.6 %, with panleukopenia in 4.9 %. The greatest number of the registered cats had acute and subacute course of chlamydia. In the acute course, there was an increase in temperature, oppression and refusal to eat. In the majority of cases of acute, subacute and associated chlamydia, the structures of the eye were damaged. Conjunctivitis, keratitis, chemosis, uveitis, third eyelid inflammation, changes in the shape of the pupil developed. A Sphynx breed cat showed signs of acute chlamydial arthritis with pronounced inflammatory edema. The latent course was accompanied by abortions, which were mainly registered in young cats. Animals older than four years more often showed stillborn and weak kittens. The main clinical signs of chlamydia in kittens born from sick cats were eye lesions: swelling of the conjunctiva, its reddening and secretion of a significant amount of brown purulent exudate with the viscosity of the catarrh and as a consequence of eyelid adhesion and complications as ankyloblepharon or symblepharon. Submandibular and other lymph nodes in the head area were significantly enlarged. Often pus dried up near the mouth or nostrils. Pus crusts accumulated in the nasal cavity and prevented breathing. Gastrointestinal tract disorders were observed in some cases. Part of the affected litter died within the first three days after birth. Neonatal chlamydia was more often manifested by conjunctivitis, keratitis, panophthalmitis and growth retardation. On the second or third day after birth, the kittens showed weakness and lack of appetite. In two cases of neonatal chlamydia, bronchopneumonia developed. When chlamydia and infectious rhinotracheitis were associated, the structures of the eye were affected in all cases. These lesions could be different and depended on the stage of the infectious process.

Utilization of mineralized fossil water (MFW), as a by-product of oil production is a special environmental problem in oil and gas areas. In Ukraine the annual production of mineralized fossil water together with oil is estimated at about 20 million tons. Taking into account that MFW is an aby-product of oil production, they are usually placed in waste disposal sites and can contaminate environment. It was determined that toxicological properties of mineralized fossil water have not been studied so far. There is the need for study of toxicological features of the MFW which will allow to determine its hazard class and to develop some measures for safe usage of this drug for making the agriculture more ecological. Thus, in the work we have made the assessment of ecotoxicological properties of MFW as an environmentally safe substitute for agrochemicals. It was established that MFW is referred to low-toxic compounds. LD 50 for oral introduction of the compound in the bodies of white female rats is more than 21,000 mg/kg, female mice -31,000 mg/kg, male mice -more than 20,000 mg/kg; when applied to the skin of rats -more than 8000 mg/kg. The value of LD 50 when applied to the inhalation routes of entry for female rats is more than 5000 mg/kg. It was justified that by oral route of entry and skin penetration the MFWs belong to the IV class of danger, and by inhalation route of entry -to III class. It was also determined that MFW does not have an irritating effect on the skin and mucous membranes of the eyes. There is also no sensitizing effect on the body of guinea pigs. Studies have shown that the cumulative properties of MFW are weakly represented. The accumulation coefficient is greater than 5. At a dose of 4260 mg/kg MFW has cardiotoxic effect, it affects the process of erythropoiesis and increases filtration and excretory function of the kidneys. Ineffective dose for rats in a subchronic experiment is 2130 mg/kg. These results of ecotoxicological study of MFW make it possible to confirm that its usage is safe in the agricultural system, especially as environmentally friendly organo-mineral fertilizer, herbicide, and fungicide.