A checklist of ascomycetous microfungi of the Nuratau Nature Reserve (Nuratau Mountains, Uzbekistan) was compiled for the first time as a result of field research conducted in 2009–2017. In total, 197 species, 3 varieties and 51 forms of micromycetes belonging to 66 genera and 30 families have been identified. Among them 19 species (Asteromella tanaceti, Camarosporium achilleae, Diplocarpon alpestre, Diplodia celtidis, Hendersonia ephedrae, Mycosphaerella artemisiae, Neopseudocercosporella capsellae, Phoma hedysari, P. mororum, Phyllosticta prostrata, P. silenes, P. trifolii, Ramularia trifolii, Rhabdospora eremuri, Selenophoma nebulosa, Septoria cyperi, S. dauci, S. ranunculacearum, S. trifolii) and one form (Erysiphe cichoracearum f. tanaceti) were found for the first time for the mycobiota of Uzbekistan. 30 species of microfungi were recorded on 31 new host plants. The most abundant species are representatives of the cosmopolitan genera Ramularia, Septoria, Erysiphe, Leveillula, Mycosphaerella, Phoma, Cytospora, Sphaerotheca, Phyllosticta and Marssonina. The annotated checklist includes data on host plant, location, date and collection number of every species.